Detection of terminal mismatches on DNA duplexes in homogeneous assays or with immobilized probes
| Autor: | Yves Aubert, Marcel Chassignol, Ulysse Asseline, Victoria Roig |
|---|---|
| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Base Pair Mismatch
010405 organic chemistry Chemistry DNA Mutational Analysis General Medicine 010402 general chemistry Thiazole orange 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences chemistry.chemical_compound Terminal (electronics) Homogeneous Genetics Molecular Medicine DNA Probes Oligonucleotide Probes DNA Fluorescent Dyes |
| Zdroj: | Nucleosides, Nucleotides and Nucleic Acids Nucleosides, Nucleotides and Nucleic Acids, Taylor & Francis, 2007, 26, pp.1669-1672 |
| ISSN: | 1525-7770 1532-2335 |
| Popis: | We recently reported the design of new fluorescent oligo-2'-deoxyribonucleotides (FODNs) for the detection of terminal mismatches on DNA duplexes in homogeneous assays. We now report the validation of this method in homogeneous assays with other sequences and the feasibility of the detection of terminal mismatches with immobilized FODNs. In all cases studied, the mismatched duplexes were more fluorescent than the perfect ones and results confirmed that the discrimination factor is sequence-dependent. |
| Databáze: | OpenAIRE |
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