Evaluation of an Ion-Exchange HPLC Device for HbA1c Measurement
| Autor: | Anthony A. Killeen, Vicky Makky, Amy K. Saenger, Ghaith Altawallbeh, Jennifer M. Peters |
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| Rok vydání: | 2019 |
| Předmět: |
Glycated Hemoglobin
Spectrum analyzer Ion exchange hplc Chromatography Chemistry Hemoglobin variants Reproducibility of Results 030209 endocrinology & metabolism Normal hemoglobin General Medicine 030204 cardiovascular system & hematology Hemoglobin A1c measurement Chromatography Ion Exchange High-performance liquid chromatography Sample stability 03 medical and health sciences 0302 clinical medicine Method comparison Humans Chromatography High Pressure Liquid |
| Zdroj: | The journal of applied laboratory medicine. 5(4) |
| ISSN: | 2576-9456 |
| Popis: | Background The ADAMS™ HA-8180V is the 8th generation of a fully automated ion-exchange HPLC system from ARKRAY, and the first to be released onto the US market. We evaluated the HA-8180V, for routine hemoglobin A1c measurement in comparison with the Roche Cobas c501, the Tosoh G8 analyzer for normal hemoglobin, and with the Trinity analyzer for hemoglobin variants. Methods The analytical performance (linearity, precision, carryover, and sample stability) was assessed based on the Clinical and Laboratory Standards Institute (CLSI) and manufacturer guidelines. A comparison of the HA-8180V against two major analytical methods was performed for 100 whole blood samples. HA-8180V variant mode was also compared against the Trinity ultra2 A1c analyzer for 50 samples containing hemoglobin variants (HbC 14, HbS 14, HbD 12, and HbE 10). Results The within-run and total CVs were Conclusion The eighth generation ADAMS HA-8180V A1c analyzer demonstrated high analytical performance adequate for routine clinical use. |
| Databáze: | OpenAIRE |
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