OsZIP7 functions in xylem loading in roots and inter-vascular transfer in nodes to deliver Zn/Cd to grain in rice
| Autor: | Yuxing Zhu, Longtao Tan, Jiurong Wang, Can Peng, Tony Fan, Caiyan Chen, Liang Sun |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Mutant Biophysics chemistry.chemical_element Zinc Genes Plant Models Biological Plant Roots Biochemistry Gene Knockout Techniques 03 medical and health sciences 0302 clinical medicine Hydroponics Gene Expression Regulation Plant Xylem Parenchyma Cation Transport Proteins Molecular Biology Plant Proteins Cadmium Oryza sativa Chemistry food and beverages Oryza Cell Biology Plants Genetically Modified Vascular bundle Recombinant Proteins 030104 developmental biology 030220 oncology & carcinogenesis Brown rice Plant Vascular Bundle |
| Zdroj: | Biochemical and Biophysical Research Communications. 512:112-118 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2019.03.024 |
| Popis: | Rice has lower zinc (Zn) but higher cadmium (Cd) content in grains than other staple crops. Understanding the molecular mechanisms involved in Zn and Cd transportation could benefit homeostatic control, facilitating optimisation of Zn and Cd levels to provide maximum nutrition and safety. In this study, we functionally characterised in planta the rice (Oryza sativa) transporter OsZIP7, which encodes a plasma membrane-localised protein with influx transport activity for both Zn and Cd. OsZIP7 was expressed in parenchyma cells of vascular bundles in roots and nodes. OsZIP7 knockout resulted in retention of Zn and Cd in roots and basal nodes, which hindered their upward delivery to upper nodes and brown rice. And a short-term labelling experiment with the stable 67Zn isotope showed that Zn was distributed toward roots and basal regions and away from leaves in the mutant compared with wild-type rice. Thus, OsZIP7 plays an integral role in xylem loading in roots and inter-vascular transfer in nodes to preferentially deliver Zn and Cd to developing tissues and rice grains. |
| Databáze: | OpenAIRE |
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