Detection of myelin autoantibodies: evaluation of an assay system for diagnosis of multiple sclerosis in differentiation from other central nervous system diseases
| Autor: | Markus Langkamp, Lutz Pridzun, Stephanie C. Hörnig, Malte E. Kornhuber, Marion Kirschner, Joachim B. Hörnig |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Proteolipid protein 1 Multiple Sclerosis Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Immunoglobulin G Diagnosis Differential Myelin Central Nervous System Diseases medicine Animals Humans Autoantibodies biology Multiple sclerosis Biochemistry (medical) Autoantibody Reproducibility of Results General Medicine medicine.disease Myelin basic protein medicine.anatomical_structure Immunoglobulin M Immunology biology.protein Disease Progression Cattle Antibody |
| Zdroj: | Clinical chemistry and laboratory medicine. 47(11) |
| ISSN: | 1437-4331 |
| Popis: | Background: Multiple sclerosis (MS) is a frequent and often severe autoimmune disease of the central nervous system. We describe a newly developed enzyme-linked immunosorbent assay (ELISA)-based test system for the assessment of neuronal autoantibodies in serum and cerebrospinal fluid (CSF). This tool could help define autoimmune status and thus be a potential means of therapeutic surveillance. Methods: We used an assay system (ELISA, E100, Mediagnost) based on purified bovine antigens [myelin-oligodendrocyte glycoprotein (MOG), myelin basic protein (MBP), myelin-associated glycoprotein (MAG), proteolipid protein (PLP) and alpha-B-crystalline (CRY)] antibodies for the measurement of specific immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies. Assay characteristics and preliminary validation were conducted by measurement of serum and CSF samples from 41 MS patients and 128 patients with other neurological diseases (OND). Results: We measured the inter- (17.8/10.1%) and intra-assay variability (5.5/6.7%); linearity (1:250– 1:16,000), and specificity of IgG and IgM. We demonstrate that by the results of this test system MS patients can be differentiated from patients with OND. Conclusions: The ELISA kit we evaluated is suitable for the measurement of neuronal autoantibodies. The initial validation demonstrates its potential use in the differential diagnosis of central neuronal system diseases. Clin Chem Lab Med 2009;47:1395–400. |
| Databáze: | OpenAIRE |
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