Increased number of mast cells in epicardial adipose tissue of cardiac surgery patients with coronary artery disease
| Autor: | Martin Haluzik, Peter Ivak, Ivan Netuka, Michal Lips, A Pierzynová, Tomáš Kučera, Jaroslav Lindner, Helena Kratochvilova, T Kotulák, K Rozsívalová |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Physiology Adipose tissue Tryptase Inflammation Coronary Artery Disease 030204 cardiovascular system & hematology Coronary artery disease 03 medical and health sciences 0302 clinical medicine Humans Medicine Mast Cells Cardiac Surgical Procedures Aged Aged 80 and over biology business.industry CD117 Myocardium Chymase Articles General Medicine Middle Aged medicine.disease Mast cell Coronary arteries 030104 developmental biology medicine.anatomical_structure Adipose Tissue biology.protein Female medicine.symptom business Pericardium Biomarkers |
| Zdroj: | Physiol Res |
| ISSN: | 1802-9973 0862-8408 |
| Popis: | Chronic inflammation of adipose tissue is associated with the pathogenesis of cardiovascular diseases. Mast cells represent an important component of the innate defense system of the organism. In our work, we quantified mast cell number in epicardial adipose tissue (EAT), subcutaneous adipose tissue (SAT), and right atrial myocardium (RA) in patients undergoing open heart surgery (n=57). Bioptic samples of EAT (n=44), SAT (n=42) and RA (n=17) were fixed by 4 % paraformaldehyde and embedded into paraffin. An anti-mast cell tryptase antibody was used for immunohistochemical detection and quantification of mast cells. We also demonstrated immunohistochemically the expression of CD117 and chymase markers. In EAT of patients with coronary artery disease (CAD), higher incidence of mast cells has been found compared to patients without CAD (3.7±2.6 vs. 2.1±1.2 cells/mm(2)). In SAT and RA, there was no difference in the number of mast cells in CAD and non-CAD patients. Mast cells in SAT, EAT and RA expressed CD117 and chymase. An increased incidence of mast cells in EAT of CAD patients may indicate the specific role of these inflammatory cells in relation to EAT and coronary arteries affected by atherosclerosis. |
| Databáze: | OpenAIRE |
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