The Arabidopsis circadian clock protein PRR5 interacts with and stimulates ABI5 to modulate abscisic acid signaling during seed germination
| Autor: | Xiao Han, Yanjuan Jiang, Milian Yang, Yanru Hu, Jiajia Yang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine AcademicSubjects/SCI01280 Circadian clock Arabidopsis Germination Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Arabidopsis thaliana Circadian rhythm Transcription factor Abscisic acid Research Articles AcademicSubjects/SCI01270 biology AcademicSubjects/SCI02288 Arabidopsis Proteins AcademicSubjects/SCI02287 organic chemicals AcademicSubjects/SCI02286 fungi food and beverages Cell Biology biology.organism_classification Cell biology Repressor Proteins Basic-Leucine Zipper Transcription Factors 030104 developmental biology chemistry Seeds Function (biology) Abscisic Acid Signal Transduction Transcription Factors 010606 plant biology & botany |
| Zdroj: | The Plant Cell Plant Cell |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1093/plcell/koab168 |
| Popis: | Seed germination and postgerminative growth require the precise coordination of multiple intrinsic and environmental signals. The phytohormone abscisic acid (ABA) suppresses these processes in Arabidopsis thaliana and the circadian clock contributes to the regulation of ABA signaling. However, the molecular mechanism underlying circadian clock-mediated ABA signaling remains largely unknown. Here, we found that the core circadian clock proteins PSEUDO-RESPONSE REGULATOR5 (PRR5) and PRR7 physically associate with ABSCISIC ACID-INSENSITIVE5 (ABI5), a crucial transcription factor of ABA signaling. PRR5 and PRR7 positively modulate ABA signaling redundantly during seed germination. Disrupting PRR5 and PRR7 simultaneously rendered germinating seeds hyposensitive to ABA, whereas the overexpression of PRR5 enhanced ABA signaling to inhibit seed germination. Consistent with this, the expression of several ABA-responsive genes is upregulated by PRR proteins. Genetic analysis demonstrated that PRR5 promotes ABA signaling mainly dependently on ABI5. Further mechanistic investigation revealed that PRR5 stimulates the transcriptional function of ABI5 without affecting its stability. Collectively, our results indicate that these PRR proteins function synergistically with ABI5 to activate ABA responses during seed germination, thus providing a mechanistic understanding of how ABA signaling and the circadian clock are directly integrated through a transcriptional complex involving ABI5 and central circadian clock components. PRR proteins physically interact with and stimulate ABI5 transcription factor to integrate circadian clock and ABA signaling during seed germination in Arabidopsis. |
| Databáze: | OpenAIRE |
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