Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Autor: Shugo Sasaki, Julia Ast, Daniela Nasteska, Fiona B. Ashford, Andrea Bacon, Zania Stamataki, Tom Podewin, Alejandra Tomas, Nicholas H. F. Fine, David J. Hodson, Giuseppe D'Agostino, Maria Lucey, Elisa D’Este, Christopher A. Reissaus, Amelia K. Linnemann, Stefan Trapp, Zsombor Koszegi, Johannes Broichhagen, Ben Jones, Kai Johnsson, Daniel I. Brierley, Francis C. Lynn, Benoit Hastoy, Anastasia Arvaniti, Frank Reimann, Davide Calebiro
Přispěvatelé: Ast, Julia [0000-0002-0039-4762], Fine, Nicholas H F [0000-0003-2343-8534], Nasteska, Daniela [0000-0002-8996-5102], Stamataki, Zania [0000-0003-3823-4497], Sasaki, Shugo [0000-0002-3696-7809], Brierley, Daniel I [0000-0002-4360-2648], Hastoy, Benoit [0000-0003-1244-7857], D'Agostino, Giuseppe [0000-0002-3502-4251], Reimann, Frank [0000-0001-9399-6377], Lynn, Francis C [0000-0001-9318-1063], Linnemann, Amelia K [0000-0001-7356-4876], Calebiro, Davide [0000-0002-3811-1553], Trapp, Stefan [0000-0003-0665-4948], Johnsson, Kai [0000-0002-8002-1981], Podewin, Tom [0000-0002-1632-5104], Broichhagen, Johannes [0000-0003-3084-6595], Hodson, David J [0000-0002-8641-8568], Apollo - University of Cambridge Repository, Medical Research Council (MRC), Fine, Nicholas H. F. [0000-0003-2343-8534], Brierley, Daniel I. [0000-0002-4360-2648], D’Agostino, Giuseppe [0000-0002-3502-4251], Lynn, Francis C. [0000-0001-9318-1063], Linnemann, Amelia K. [0000-0001-7356-4876], Hodson, David J. [0000-0002-8641-8568], Fine, Nicholas HF [0000-0003-2343-8534]
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Models
Molecular
genetic structures
Lydia Becker Institute
123
Human Embryonic Stem Cells
96
General Physics and Astronomy
96/33
MOUSE
14
59
Optical imaging
631/92/96
chemistry.chemical_compound
Mice
0302 clinical medicine
Tissue Distribution
631/80/2373/2238
14/19
Super-resolution microscopy
EXPRESSING CELLS
Receptor
lcsh:Science
Peptide sequence
PHARMACOLOGY
Mice
Knockout
Multidisciplinary
Molecular Structure
Endocrine system and metabolic diseases
Brain
3. Good health
Multidisciplinary Sciences
631/1647/245/2226
Science & Technology - Other Topics
64/60
Signal transduction
psychological phenomena and processes
hormones
hormone substitutes
and hormone antagonists
Signal Transduction
endocrine system
Fluorophore
Science
BETA
BIOLOGY
behavioral disciplines and activities
General Biochemistry
Genetics and Molecular Biology
Article
Glucagon-Like Peptide-1 Receptor
38
Cell Line
03 medical and health sciences
Islets of Langerhans
14/34
ResearchInstitutes_Networks_Beacons/lydia_becker_institute_of_immunology_and_inflammation
GLP-1 RECEPTOR
Animals
Humans
Amino Acid Sequence
Glucagon-like peptide 1 receptor
G protein-coupled receptor
Fluorescent Dyes
RELEASE
Science & Technology
45
FLUOROGENIC PROBES
HEK 293 cells
General Chemistry
Peptide Fragments
96/100
ALPHA
030104 developmental biology
HEK293 Cells
Microscopy
Fluorescence
Multiphoton
nervous system
chemistry
692/163/2743
TISSUE
13/51
14/63
Biophysics
lcsh:Q
14/69
Chemical tools
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Nature Communications
Ast, J, Arvaniti, A, Fine, N H F, Nasteska, D, Ashford, F B, Stamataki, Z, Koszegi, Z, Bacon, A, Jones, B J, Lucey, M A, Sasaki, S, Brierley, D I, Hastoy, B, Tomas, A, D'Agostino, G, Reimann, F, Lynn, F C, Reissaus, C A, Linnemann, A K, D'Este, E, Calebiro, D, Trapp, S, Johnsson, K, Podewin, T, Broichhagen, J & Hodson, D J 2020, ' Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics ', Nature Communications, vol. 11, no. 1, 467, pp. 467 . https://doi.org/10.1038/s41467-020-14309-w
ISSN: 2041-1723
DOI: 10.1038/s41467-020-14309-w
Popis: The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled receptor (GPCR) involved in metabolism. Presently, its visualization is limited to genetic manipulation, antibody detection or the use of probes that stimulate receptor activation. Herein, we present LUXendin645, a far-red fluorescent GLP1R antagonistic peptide label. LUXendin645 produces intense and specific membrane labeling throughout live and fixed tissue. GLP1R signaling can additionally be evoked when the receptor is allosterically modulated in the presence of LUXendin645. Using LUXendin645 and LUXendin651, we describe islet, brain and hESC-derived β-like cell GLP1R expression patterns, reveal higher-order GLP1R organization including membrane nanodomains, and track single receptor subpopulations. We furthermore show that the LUXendin backbone can be optimized for intravital two-photon imaging by installing a red fluorophore. Thus, our super-resolution compatible labeling probes allow visualization of endogenous GLP1R, and provide insight into class B GPCR distribution and dynamics both in vitro and in vivo.
Glucagon-like peptide-1 receptor is an important regulator of appetite and glucose homeostasis. Here the authors describe super-resolution microscopy and in vivo imaging compatible fluorescent probes, which reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics in islets and brain.
Databáze: OpenAIRE
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