Evaluation of the Effect of Strigolactones and Synthetic Analogs on Fungi
| Autor: | Mara Novero, Luisa Lanfranco, Valentina Fiorilli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Strigolactones integumentary system biology Hypha Inoculation Arbuscular mycorrhizal fungi Fungi Germination In vitro growth Strigolactones fungi Fungi Arbuscular mycorrhizal fungi Germination Fungus biology.organism_classification 01 natural sciences Conidium 03 medical and health sciences 030104 developmental biology Botany Spore germination Axenic In vitro growth 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071614280 |
| Popis: | Strigolactones (SLs) are components of root exudates as a consequence of active release from the roots into the soil. Notably, they have been described as stimulants of seed germination in parasitic plants and of the presymbiotic growth in arbuscular mycorrhizal (AM) fungi, which are a crucial component of the plant root beneficial microbiota. SLs have therefore the potential to influence other microbes that proliferate in the soil around the roots and may interact with plants. A direct effect of SL analogs on the in vitro growth of a number of saprotrophic or plant pathogenic fungi was indeed reported.Here we describe a standardized method to evaluate the effect of SLs or their synthetic analogs on AM and filamentous fungi. For AM fungi, we propose a spore germination assay since it is more straightforward than the hyphal branching assay and it does not require deep expertise and skills. For filamentous fungi that can grow in axenic cultures, we describe the assay based on SLs embedded in the solid medium or dissolved in liquid cultures where the fungus is inoculated to evaluate the effect on growth, hyphal branching or conidia germination. These assays are of help to test the activity of natural SLs as well as of newly designed SL analogs for basic and applied research. |
| Databáze: | OpenAIRE |
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