Genetic variation and phylogenetic analysis of 22 French isolates of equine arteritis virus

Autor: Stéphan Zientara, F. Miszczak, U.B.R. Balasuriya, Peter J. Timoney, G. Fortier, Jianqiang Zhang, S. Pronost, Christine Fortier
Přispěvatelé: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Laboratoire Départemental du Calvados - Frank Duncombe, Partenaires INRAE, Virologie, École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)
Rok vydání: 2007
Předmět:
Male
040301 veterinary sciences
Molecular Sequence Data
G(L) ENVELOPE GLYCOPROTEIN
Biology
PERSISTENT INFECTION
0403 veterinary science
Arterivirus
03 medical and health sciences
Equartevirus
Semen
Phylogenetics
Virology
Genetic variation
INFECTIOUS CDNA-CLONE
Animals
MOLECULAR CHARACTERIZATION
Horses
ORFS
NEUTRALIZATION DETERMINANTS
VIRAL ARTERITIS
Lung
Phylogeny
030304 developmental biology
Genetics
0303 health sciences
Genetic diversity
Arterivirus Infections
Phylogenetic tree
Strain (biology)
LABORATORY STRAINS
Genetic Variation
Sequence Analysis
DNA
04 agricultural and veterinary sciences
General Medicine
Abortion
Veterinary
biology.organism_classification
STRUCTURAL PROTEIN
GenBank
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Female
Horse Diseases
France
MONOCLONAL-ANTIBODIES
AMINO-ACID-SEQUENCES
Zdroj: Archives of Virology
Archives of Virology, Springer Verlag, 2007, 152 (11), pp.1977-1994
ISSN: 1432-8798
0304-8608
2001-2004
DOI: 10.1007/s00705-007-1040-z
Popis: International audience; Genetic variation and phylogenetic relationships among 22 French isolates of equine arteritis virus (EAV) obtained over four breeding seasons (2001-2004) were determined by sequencing open reading frames (ORFs) 2a-7. The ORFs 2a-7 of 22 isolates differed from the prototype virulent Bucyrus strain of EAV by between 14 (99.5% identity) and 328 (88.7% identity) nucleotides, and differed from each other by between 0 (100% identity) and 346 (88.1% identity) nucleotides, confirming genetic diversity among EAV strains circulating in France. Phylogenetic analysis based on the partial ORF5 sequences (nucleotides 11296-11813) of 22 French isolates and 216 additional EAV strains available in GenBank clustered the global isolates of EAV into two distinct groups: North American and European. The latter could be further divided into two large subgroups: European subgroup 1 (EU-1) and European subgroup 2 (EU-2). Phylogenetic analysis based on 100 EAV ORF3 sequences yielded similar results. Of the 22 French EAV isolates, the 11 isolates obtained before January 28, 2003 clustered with either the EU-1 (9 isolates) or EU-2 (2 isolates) subgroup. In contrast, by the criteria used in this study, the 11 isolates obtained after January 30, 2003 belong to the North American group, strongly suggesting that these strains were recently introduced into France.
Databáze: OpenAIRE
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