Real-time genomic investigation underlying the public health response to a Shiga toxin-producing Escherichia coli O26:H11 outbreak in a nursery
| Autor: | John W. A. Rossen, E Anuka, F Alsana, A Rokney, M Baum, Itamar Grotto, Ruth Yishay, Mithila Ferdous, V. Agmon, Michael Gdalevich, Larissa Dukhan, Jacob Moran-Gilad, L Valinsky, Dana Danino |
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| Přispěvatelé: | Microbes in Health and Disease (MHD) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
paediatric Epidemiology medicine.disease_cause Disease Outbreaks fluids and secretions Israel Escherichia coli Infections Phylogeny UTILITY whole genome sequencing Shiga-Toxigenic Escherichia coli Transmission (medicine) Escherichia coli Proteins Genomics Original Papers Infectious Diseases PCR INFECTIONS Public Health Nurseries Infant haemolytic-uremic syndrome VIRULENCE FACTORS Horizontal transmission medicine.medical_specialty 030106 microbiology Biology Microbiology 03 medical and health sciences SURVEILLANCE medicine Escherichia coli Humans Typing O157 Intimin JAPAN investigation outbreak Public health Outbreak Infant Sequence Analysis DNA Virology Shigatoxin 030104 developmental biology Hemolytic-Uremic Syndrome bacteria |
| Zdroj: | Epidemiol Infect Epidemiology And Infection, 145(14), 2998-3006. Cambridge University Press |
| ISSN: | 1469-4409 0950-2688 |
| Popis: | SUMMARYShiga toxin-producingEscherichia coli(STEC) is a significant cause of gastrointestinal infection and the haemolytic-uremic syndrome (HUS). STEC outbreaks are commonly associated with food but animal contact is increasingly being implicated in its transmission. We report an outbreak of STEC affecting young infants at a nursery in a rural community (three HUS cases, one definite case, one probable case, three possible cases and five carriers, based on the combination of clinical, epidemiological and laboratory data) identified using culture-based and molecular techniques. The investigation identified repeated animal contact (animal farming and petting) as a likely source of STEC introduction followed by horizontal transmission. Whole genome sequencing (WGS) was used for real-time investigation of the incident and revealed a unique strain of STEC O26:H11 carryingstx2aand intimin. Following a public health intervention, no additional cases have occurred. This is the first STEC outbreak reported from Israel. WGS proved as a useful tool for rapid laboratory characterization and typing of the outbreak strain and informed the public health response at an early stage of this unusual outbreak. |
| Databáze: | OpenAIRE |
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