Simultaneous LC−MS/MS determination of five tripterygium pyridine alkaloids in dog plasma and its application to their pharmacokinetic study after oral administration of tripterygium glycosides tablets
| Autor: | Bin Di, Mengxiang Su, Jin-fang Shi, Da-song Yang, Min Song, Taijun Hang |
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| Rok vydání: | 2015 |
| Předmět: |
Pyridines
Tripterygium Formic acid Clinical Biochemistry Administration Oral Sensitivity and Specificity Biochemistry Analytical Chemistry chemistry.chemical_compound Alkaloids Dogs Drug Stability Pharmacokinetics Tandem Mass Spectrometry Oral administration Ammonium formate Animals chemistry.chemical_classification Chromatography biology Selected reaction monitoring Reproducibility of Results Glycoside Cell Biology General Medicine Triptolide biology.organism_classification chemistry Linear Models Chromatography Liquid Drugs Chinese Herbal Tablets |
| Zdroj: | Journal of Chromatography B. 990:31-38 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2015.03.011 |
| Popis: | A sensitive and selective liquid chromatography tandem mass spectrometric method was developed and validated for the simultaneous determination of five pyridine alkaloids contained in tripterygium glycosides tablets (triptolide, wilforine, wilforgine, wilfording and wilfortrine) in dog plasma. The analysis was carried out on a Sepax GP-Phenyl column using a mixture of methanol and 10mmol/L ammonium formate buffer solution containing 0.1% formic acid (75:25, v/v) as the mobile phase pumped at a flow-rate of 1.0mL/min. All MS data were obtained in the positive ESI mode with selective multiple reaction monitoring of ion transitions. The method was fully validated to be accurate and precise with a linear range of 0.2-1000ng/mL for triptolide and 0.05-1000ng/mL for the other four pyridine alkaloids. The intra-day and inter-day precisions (relative standard deviation, RSD, %) were within 10.6% and 14.0%, respectively, and the relative error (RE, %) were all less than 13.1%. The method was successfully applied to multi-components pharmacokinetic study of the five pyridine alkaloids in beagle dogs after a single oral administration of 3mg/kg and 30mg/kg tripterygium glycosides tablets, respectively, and a multiple oral administration of 30mg/kg for 6 consecutive days. |
| Databáze: | OpenAIRE |
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