Label-free enrichment of fate-biased human neural stem and progenitor cells

Autor: Alan Y.L. Jiang, Tayloria N.G. Adams, Nicolo S. Mendoza, Abraham P. Lee, Lisa A. Flanagan, Do-Hyun Lee, Clarissa C. Ro
Rok vydání: 2019
Předmět:
Cellular differentiation
Cell
02 engineering and technology
Biosensing Techniques
Cell Separation
Regenerative Medicine
01 natural sciences
Analytical Chemistry
Neural Stem Cells
Stem Cell Research - Nonembryonic - Human
Lab-On-A-Chip Devices
Electrochemistry
Nanotechnology
Neurons
General Medicine
Equipment Design
Cell sorting
021001 nanoscience & nanotechnology
Cell biology
Oligodendroglia
medicine.anatomical_structure
Membrane capacitance
Neurological
Development of treatments and therapeutic interventions
Astrocyte
0210 nano-technology
Biotechnology
Cell type
Bioinformatics
Dielectrophoresis
Biomedical Engineering
Biophysics
Neural stem and progenitor cell
Biology
Electric Capacitance
Article
Cell Line
medicine
Humans
Progenitor cell
5.2 Cellular and gene therapies
010401 analytical chemistry
Neurosciences
Stem Cell Research
Brain Disorders
0104 chemical sciences
Electrophysiology
Microfluidic
Astrocytes
Zdroj: Biosens Bioelectron
ISSN: 1873-4235
Popis: Human neural stem and progenitor cells (hNSPCs) have therapeutic potential to treat neural diseases and injuries since they provide neuroprotection and differentiate into astrocytes, neurons, and oligodendrocytes. However, cultures of hNSPCs are heterogeneous, containing cells linked to distinct differentiated cell fates. HNSPCs that differentiate into astrocytes are of interest for specific neurological diseases, creating a need for approaches that can detect and isolate these cells. Astrocyte-biased hNSPCs differ from other cell types in electrophysiological properties, namely membrane capacitance, and we hypothesized that this could be used to enrich these cells using dielectrophoresis (DEP). We implemented a two-step DEP sorting scheme, consisting of analysis to define the optimal sorting frequency followed by separation of cells at that frequency, to test whether astrocyte-biased cells could be separated from the other cell types present in hNSPC cultures. We developed a novel device that increased sorting reproducibility and provided both enriched and depleted cell populations in a single sort. Astrocyte-biased cells were successfully enriched from hNSPC cultures by DEP sorting, making this the first study to use electrophysiological properties for label-free enrichment of human astrocyte-biased cells. Enriched astrocyte-biased human cells enable future experiments to determine the specific properties of these important cells and test their therapeutic efficacy in animal models of neurological diseases.
Databáze: OpenAIRE
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