Isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids
| Autor: | V.D. Bokkenheuser, William G. Kelly, Dehazya P, J. Winter |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Microbiological culture
medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Feces medicine Humans Eubacterium Anaerobiosis Desoxycorticosterone Escherichia coli Human feces Ecology biology Obligate anaerobe biology.organism_classification Isolation (microbiology) Anti-Bacterial Agents Fecal coliform Biochemistry Pregnanediol Steroid 21-Hydroxylase Food Science Biotechnology Research Article |
| Zdroj: | Applied and environmental microbiology. 34(5) |
| ISSN: | 0099-2240 |
| Popis: | It has been known for a decade that human intestinal flora include organisms capable of 21-dehydroxylating corticoids. Yet the identity of the organisms synthesizing 21-dehydroxylase has remained unknown. Using diluted human feces, we determined the prevalence of colonies of 21-dehydroxylating organisms on a variety of media. Isolation from the medium of colonies with the highest prevalence yielded an obligate anaerobe capable of 21-dehydroxylating deoxycorticosterone and tetrahydrodeoxycorticosterone. This transformation could be carried out in a prereduced medium by the microbial culture alone or in an aerobic medium reduced by growth of Escherichia coli. The culture shares many characteristics with Eubacterium lentum, the neotype strain of which elaborated both 21-dehydroxylase and 3alpha-hydroxysteroid dehydrogenase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|