Selective contrast agents with potential to the earlier detection of tumors: Insights on synthetic pathways, physicochemical properties and performance in MRI assays

Autor: María Gabriela Montiel Schneider, Marcela Beatriz Fernández van Raap, Claudia Gentili, Diego Muraca, María Julia Martín, D. F. Coral, Verónica Leticia Lassalle
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Surface Properties
Gadolinium
Contrast Media
chemistry.chemical_element
Nanoparticle
02 engineering and technology
010402 general chemistry
Human colon cancer cells
Ferric Compounds
01 natural sciences
Contrast agents
purl.org/becyt/ford/1 [https]
chemistry.chemical_compound
Colloid and Surface Chemistry
HUMAN COLON CANCER CELLS
Neoplasms
purl.org/becyt/ford/1.4 [https]
Humans
Particle Size
Physical and Theoretical Chemistry
Cytotoxicity
Magnetite nanoparticles
Prussian blue
Otras Ciencias Químicas
In vitro toxicology
Ciencias Químicas
Surfaces and Interfaces
General Medicine
Química
021001 nanoscience & nanotechnology
Ascorbic acid
Magnetic Resonance Imaging
0104 chemical sciences
chemistry
Cancer cell
Biophysics
ASCORBIC ACID
CONTRAST AGENTS
0210 nano-technology
MAGNETITE NANOPARTICLES
Iron oxide nanoparticles
CIENCIAS NATURALES Y EXACTAS
Biotechnology
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Magnetic iron oxide nanoparticles (MNPs) have been prepared and stabilized with three organic acids (tartaric, malic and ascorbic) in order to obtain biocompatible and water dispersible MNPs with potential to bind specifically to tumoral cancer cells. An in deep characterization was performed aiming to verify the presence and effect of the coating and stabilizer on MNPs surface. Besides the mechanisms followed by the different acids to bind MNPs were elucidated and used to justify the differences in the physicochemical properties of each formulation. Data related to characterization revealed that MNPs coated with ascorbic acid (MNPs-AA) resulted the most suitable in terms of their size, surface charge and stability along the time. Besides, ascorbic acid may be recognized by GLUTs receptors that are overexpressed in several kinds of tumoral cells. Therefore, MNPs-AA was selected to explore its performance in both MRI and in vitro assays using human colon cancer cells HCT 116. MRI experiments were performed in clinical equipment using a series of aqueous dispersions of MNPs-AA that were evaluated as T2 contrast agent. The T2- weighted images obtained as well as the calculated r2, indicated that MNPs-AA could act as efficient T2 contrast agent for MRI. Regarding in vitro assays, MNPs-AA did not alter the cellular function neither exert cytotoxicity using the three explored doses. The internalization of the nanoparticles on the cellular structure was confirmed quanti and qualitatively using atomic absorption spectroscopy and Prussian blue techniques respectively. From these results, it emerges that ascorbic acid coated-magnetite nanoparticles may be used as alternative contrast agent to avoid or minimize some toxicological issues related to the widely used gadolinium.
Instituto de Física La Plata
Databáze: OpenAIRE
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