Novel large-scale chromosomal transfer in Bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation
| Autor: | Sheila Patrick, Kevin Tang, Yaligara Veeranagouda, Renata F. Boente, Hannah M. Wexler, Fasahath Husain, Garry W. Blakely |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene Transfer Horizontal 030106 microbiology Adaptation Biological Host Adaptation Microbiology Bacteroides fragilis 03 medical and health sciences chemistry.chemical_compound Intergenic region Humans Gene restriction modification system Medicine(all) Genetics Recombination Genetic biology Polysaccharides Bacterial Chromosome Pan-genome General Medicine Chromosomes Bacterial horizontal transmission biology.organism_classification Bacteroides Infections polysaccharide capsules Antigenic Variation Gastrointestinal Microbiome 030104 developmental biology chemistry bacteroides fragilis Microbe-Niche Interactions DNA Transposable Elements Restriction modification system Bacteroides DNA Research Article |
| Zdroj: | Microbial Genomics Husain, F, Tang, K, Veeranagouda, Y, Boente, R, Patrick, S, Blakely, G & Wexler, H M 2017, ' Novel large-scale chromosomal transfer in Bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation ', Microbial Genomics, vol. 2017, no. 3 . https://doi.org/10.1099/mgen.0.000136 Husain, F, Tang, K, Veeranagouda, Y, Boente, R, Patrick, S, Blakely, G & Wexler, H M 2017, ' Novel large-scale chromosomal transfer in bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation ', Microbial Genomics, vol. 3, no. 11, 000136 . https://doi.org/10.1099/mgen.0.000136 |
| ISSN: | 2057-5858 |
| Popis: | Bacteroides fragilis, an important component of the human gastrointestinal microbiota, can cause lethal extra-intestinal infection upon escape from the gastrointestinal tract. We demonstrated transfer and recombination of large chromosomal segments from B. fragilis HMW615, a multidrug resistant clinical isolate, to B. fragilis 638R. In one example, the transfer of a segment of ~435 Kb/356 genes replaced ~413 Kb/326 genes of the B. fragilis 638R chromosome. In addition to transfer of antibiotic resistance genes, these transfers (1) replaced complete divergent polysaccharide biosynthesis loci; (2) replaced DNA inversion-controlled intergenic shufflons (that control expression of genes encoding starch utilization system outer membrane proteins) with more complex, divergent shufflons; and (3) introduced additional intergenic shufflons encoding divergent Type 1 restriction/modification systems. Conjugative transposon-like genes within a transferred segment and within a putative integrative conjugative element (ICE5) ~45 kb downstream from the transferred segment both encode proteins that may be involved in the observed transfer. These data indicate that chromosomal transfer is a driver of antigenic diversity and nutrient adaptation in Bacteroides that (1) contributes to the dissemination of the extensive B. fragilis pan-genome, (2) allows rapid adaptation to a changing environment and (3) can confer pathogenic characteristics to host symbionts. |
| Databáze: | OpenAIRE |
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