Association Between HMGB1 and Thrombogenesis in a Hyperlipaemia-induced Microminipig Model of Atherosclerosis
| Autor: | Hiroaki Kawaguchi, Akihide Tanimoto, Tomonobu Yamada, Tomoka Nagasato, Satoru Kake, Naoki Miura, Ikuro Maruyama, Takashi Ito |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
medicine.medical_specialty Normal diet Inflammation chemical and pharmacologic phenomena Hyperlipidemias Matrix metalloproteinase HMGB1 Diet High-Fat General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animal model Internal medicine medicine Animals Thrombus HMGB1 Protein Pharmacology biology business.industry Cholesterol medicine.disease Atherosclerosis Endocrinology chemistry 030220 oncology & carcinogenesis biology.protein medicine.symptom business Dietary Cholesterol Research Article |
| Zdroj: | In Vivo |
| ISSN: | 1791-7549 |
| Popis: | Background/aim An appropriate animal model is essential to investigate the relationship between inflammation, atherosclerosis, and thrombogenesis, and the development of preventive measures and therapies for atherosclerosis. Materials and methods Atherosclerosis was induced in Microminipigs (MMPs) using a high-fat diet. We assessed high mobility group box 1 (HMGB1) expression levels and measured thrombus formation using a Total Thrombus Formation Analysis System (T-TAS). MMPs were divided into a normal diet (control) group and four high-fat diet groups, with differing amounts of cholesterol. After 8 weeks, blood was collected for analysis. Results HMGB1 levels increased with increasing dietary cholesterol, and a negative correlation was found between HMGB1 levels and thrombus formation time. Conclusion T-TAS is useful in the assessment of thrombogenesis in MMPs and HMGB1 is associated with thrombus formation. |
| Databáze: | OpenAIRE |
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