Molecular Analysis of East African Lumpy Skin Disease Viruses Reveals a Mixed Isolate with Features of Both Vaccine and Field Isolates
| Autor: | Angelika Loitsch, Tesfaye Rufael Chibssa, Yazeed Abd Elraouf, Melaku Sombo, Francisco J. Berguido, Charles Euloge Lamien, Jacqueline Kasiiti Lichoti, Giovanni Cattoli, Adama Diallo, Mesfin Sahle, Tirumala B. K. Settypalli, Yang Liu, Reingard Grabherr, Tajelser Adam |
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| Přispěvatelé: | International Atomic Energy Agency [Vienna] (IAEA), National Animal Health Diagnostic and Investigation Center (NAHDIC), Universität für Bodenkultur Wien [Vienne, Autriche] (BOKU), Ministry of Agriculture, Livestock and Fisheries [Kenya], Ministry of Livestock, Fisheries and Ranges, Ankara Üniversitesi, Joint FAO/IAEA Programme - Nuclear Techniques in Food and Agriculture, Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO)-International Atomic Energy Agency [Vienna] (IAEA), Austrian Agency for Health and Food Safety (AGES), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut Sénégalais de Recherches Agricoles [Dakar] (ISRA), This study was supported by the VETLAB network initiative of the Joint FAO/IAEA Division, funded through the African Renaissance and International Cooperation Fund of South Africa and the Peaceful Uses Initiatives (PUI) funded by Japan and the United States of America |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 040301 veterinary sciences QH301-705.5 [SDV]Life Sciences [q-bio] Biology RPO30 gene Microbiology Virus Article 0403 veterinary science EEV glycoprotein 03 medical and health sciences Lumpy skin disease Virology Genetic variation medicine LSDV B22R gene GPCR gene Biology (General) Pathogen Gene Epizootic Genetic diversity 04 agricultural and veterinary sciences medicine.disease Lumpy skin disease virus 3. Good health 030104 developmental biology |
| Zdroj: | Microorganisms Microorganisms, Vol 9, Iss 1142, p 1142 (2021) Microorganisms, MDPI, 2021, 9 (6), ⟨10.3390/microorganisms9061142⟩ Volume 9 Issue 6 |
| ISSN: | 2076-2607 |
| Popis: | International audience; Lumpy skin disease (LSD), an economically significant disease in cattle caused by lumpy skin disease virus (LSDV), is endemic to nearly all of Africa. Since 2012, LSDV has emerged as a significant epizootic pathogen given its rapid spread into new geographical locations outside Africa, including the Middle East, Eastern Europe, and Asia. To assess the genetic diversity of LSDVs in East Africa, we sequenced and analyzed the RPO30 and GPCR genes of LSDV in twenty-two archive samples collected in Ethiopia, Kenya, and Sudan before the appearance of LSD in the Middle East and its incursion into Europe. We compared them to publicly available sequences of LSDVs from the same region and those collected elsewhere. The results showed that the East African field isolates in this study were remarkably similar to each other and to previously sequenced field isolates of LSDV for the RPO30 and GPCR genes. The only exception was LSDV Embu/B338/2011, a field virus collected in Kenya, which displayed mixed features between the LSDV Neethling vaccine and field isolates. LSDV Embu/B338/2011 had the same 12-nucleotide insertion found in LSDV Neethling and KS-1 vaccines. Further analysis of the partial EEV glycoprotein, B22R, RNA helicase, virion core protein, NTPase, and N1R/p28-like protein genes showed that LSDV Embu/B338/2011 differs from previously described LSDV variants carrying the 12-nucleotide insertion in the GPCR gene. These findings highlight the importance of the constant monitoring of genetic variation among LSDV isolates. |
| Databáze: | OpenAIRE |
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