Combined walking exercise and alkali therapy in patients with CKD4-5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression
| Autor: | George Kosmadakis, Alan Bevington, Emma L. Watson, Karen Molyneux, João L. Viana, Izabella Z.A. Pawluczyk, Alice C. Smith, John Feehally, Michael Mulheran, Susan M. Shirreffs, Ronald J. Maughan, J. Brown, Christopher W. McIntyre, Nicolette C. Bishop, Stephen G. John, Paul J. Owen |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Anabolism Physiology Bicarbonate Ubiquitin-Protein Ligases Muscle Proteins Walking Tripartite Motif Proteins chemistry.chemical_compound Physiology (medical) Internal medicine medicine Aerobic exercise Humans Orthopedics and Sports Medicine Lactic Acid Amino Acids Renal Insufficiency Chronic Muscle Skeletal Acidosis Aged Aged 80 and over business.industry Catabolism Public Health Environmental and Occupational Health Skeletal muscle Metabolic acidosis General Medicine Middle Aged medicine.disease Combined Modality Therapy Exercise Therapy Bicarbonates medicine.anatomical_structure Endocrinology chemistry Lactic acidosis Female medicine.symptom business Proto-Oncogene Proteins c-akt |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1439-6327 |
| Popis: | Muscle-wasting in chronic kidney disease (CKD) arises from several factors including sedentary behaviour and metabolic acidosis. Exercise is potentially beneficial but might worsen acidosis through exercise-induced lactic acidosis. We studied the chronic effects of exercise in CKD stage 4-5 patients (brisk walking, 30 min, 5 times/week), and non-exercising controls; each group receiving standard oral bicarbonate (STD), or additional bicarbonate (XS) (Total n = 26; Exercising + STD n = 9; Exercising +XS n = 6; Control + STD n = 8; Control + XS n = 3). Blood and vastus lateralis biopsies were drawn at baseline and 6 months. The rise in blood lactate in submaximal treadmill tests was suppressed in the Exercising + XS group. After 6 months, intramuscular free amino acids (including the branched chain amino acids) in the Exercising + STD group showed a striking chronic depletion. This did not occur in the Exercising + XS group. The effect in Exercising + XS patients was accompanied by reduced transcription of ubiquitin E3-ligase MuRF1 which activates proteolysis via the ubiquitin-proteasome pathway. Other anabolic indicators (Akt activation and suppression of the 14 kDa actin catabolic marker) were unaffected in Exercising + XS patients. Possibly because of this, overall suppression of myofibrillar proteolysis (3-methylhistidine output) was not observed. It is suggested that alkali effects in exercisers arose by countering exercise-induced acidosis. Whether further anabolic effects are attainable on combining alkali with enhanced exercise (e.g. resistance exercise) merits further investigation. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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