Triplet-Triplet Annihilation Upconversion Based Nanosensors for Fluorescence Detection of Potassium
| Autor: | Alexandra L Dailey, Megan P. Jewell, Meredith D Greer, Kevin J. Cash |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fluid Flow and Transfer Processes
Analyte Chemistry Process Chemistry and Technology 010401 analytical chemistry Ionophore Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology Photochemistry Nile blue 01 natural sciences Fluorescence Photon upconversion 0104 chemical sciences chemistry.chemical_compound Spectrometry Fluorescence Nanosensor pH indicator Potassium Humans Nanotechnology 0210 nano-technology Instrumentation Biosensor |
| Zdroj: | ACS Sensors. 5:474-480 |
| ISSN: | 2379-3694 |
| Popis: | Typical ionophore-based nanosensors use Nile blue derived indicators called chromoionophores, which must contend with strong background absorption, autofluorescence, and scattering in biological samples that limit their usefulness. Here, we demonstrate potassium-selective nanosensors that utilize triplet-triplet annihilation upconversion to minimize potential optical interference in biological media and a pH-sensitive quencher molecule to modulate the upconversion intensity in response to changes in analyte concentration. A triplet-triplet annihilation dye pair (platinum(II) octaethylporphyrin and 9,10-diphenylanthracene) was integrated into nanosensors containing an analyte binding ligand (ionophore), charge-balancing additive, and a pH indicator quencher. The nanosensor response to potassium was shown to be reversible and stable for 3 days. In addition, the nanosensors are selective against sodium, calcium, and magnesium (selectivity coefficients in log10 units of -2.2 for calcium, -2.0 for sodium, and -2.4 for magnesium), three interfering ions found in biological samples. The lack of signal overlap between the upconversion nanosensors and GFP, a common biological fluorescent indicator, is demonstrated in confocal microscope images of sensors embedded in a bacterial biofilm. |
| Databáze: | OpenAIRE |
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