Stabilization by multipoint covalent attachment of a biocatalyst with polygalacturonase activity used for juice clarification
| Autor: | Yuly A. Ramírez Tapias, Jorge A. Trelles, Jose M. Guisan, Fernando López Gallego, Cintia W. Rivero |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Otras Biotecnología Agropecuaria Immobilized enzyme Food Handling Biotecnología Agropecuaria Polygalacturonase activity 01 natural sciences Analytical Chemistry chemistry.chemical_compound 010608 biotechnology Enzyme Stability Vitis Pectinase Enzyme purification Glyoxyl-agarose Chromatography Streptomyces halstedii ATCC 10897 biology 010405 organic chemistry Sepharose Glyoxylates Prunus domestica General Medicine Hydrogen-Ion Concentration Enzymes Immobilized Grape and plum juices Enzyme assay 0104 chemical sciences Fruit and Vegetable Juices Polygalacturonase chemistry CIENCIAS AGRÍCOLAS Biocatalysis Covalent bond Fruit Yield (chemistry) biology.protein Pectins Agarose Food Science |
| Zdroj: | Food Chemistry. 208:252-257 |
| ISSN: | 0308-8146 |
| Popis: | Derivatized-agarose supports are suitable for enzyme immobilization by different methods, taking advantage of different physical, chemical and biological conditions of the protein and the support. In this study, agarose particles were modified with MANAE, PEI and glyoxyl groups and evaluated to stabilize polygalacturonase from Streptomyces halstedii ATCC 10897. A new immobilized biocatalyst was developed using glyoxyl-agarose as support; it exhibited high performance in degrading polygalacturonic acid and releasing oligogalacturonides. Maximal enzyme activity was detected at 5 h of reaction using 0.05 g/mL of immobilized biocatalyst, which released 3 mg/mL of reducing sugars and allowed the highest product yield conversion and increased stability. These results are very favorable for pectin degradation with reusability up to 18 successive reactions (90 h) and application in juice clarification. Plum (4.7 °Bx) and grape (10.6 °Bx) juices were successfully clarified, increasing reducing sugars content and markedly decreasing turbidity and viscosity. Fil: Ramírez Tapias, Yuly Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina Fil: Gallego, Fernando López. Heterogeneous Biocatalysis Group; España. Ikerbasque; España Fil: Guisán, José M.. Institute of Catalysis and Petrochemistry; España Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina |
| Databáze: | OpenAIRE |
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