Genetically encoded fluorescent tags
| Autor: | Kurt S. Thorn |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Green Fluorescent Proteins Cell Computational biology Biology 010402 general chemistry 01 natural sciences Fluorescence Flow cytometry 03 medical and health sciences Biological property Microscopy medicine Humans Molecular Biology Fluorescent Dyes chemistry.chemical_classification medicine.diagnostic_test Biomolecule Cell Biology Flow Cytometry 0104 chemical sciences 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence chemistry Perspective Nucleic acid Protein abundance |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Genetically encoded fluorescent tags are protein sequences that can be fused to a protein of interest to render it fluorescent. These tags have revolutionized cell biology by allowing nearly any protein to be imaged by light microscopy at submicrometer spatial resolution and subsecond time resolution in a live cell or organism. They can also be used to measure protein abundance in thousands to millions of cells using flow cytometry. Here I provide an introduction to the different genetic tags available, including both intrinsically fluorescent proteins and proteins that derive their fluorescence from binding of either endogenous or exogenous fluorophores. I discuss their optical and biological properties and guidelines for choosing appropriate tags for an experiment. Tools for tagging nucleic acid sequences and reporter molecules that detect the presence of different biomolecules are also briefly discussed. |
| Databáze: | OpenAIRE |
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