Anti-inflammatory action of an alkaloid, fraction and extract from Alchornea glandulosa in mice
| Autor: | Anelise Samara Nazari Formagio, Zefa Valdevina Pereira, Candida Aparecida Leite Kassuya, Frederico Formagio-Neto, Milena Menezes Corrêa Pederiva, Claudia Andrea Lima Cardoso, Maria Helena Sarragiotto, Carla Roberta Ferreira Volobuff |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Leukocyte migration Croton Oil medicine.drug_class Administration Topical Anti-Inflammatory Agents Administration Oral Carrageenan Guanidines Anti-inflammatory law.invention Mice 03 medical and health sciences chemistry.chemical_compound Alkaloids 0302 clinical medicine law Drug Discovery medicine Caffeic acid Animals Edema Croton oil Gallic acid Pleurisy 030304 developmental biology Pharmacology 0303 health sciences Traditional medicine biology Plant Extracts Arthritis Euphorbiaceae Zymosan Alchornea glandulosa biology.organism_classification Plant Leaves chemistry 030220 oncology & carcinogenesis Female Phytotherapy Quercetin |
| Zdroj: | Journal of Ethnopharmacology. 231:66-72 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Alchornea glandulosa (Euphorbiaceae) has traditionally been used in medicine for treating immune-mediated inflammatory diseases. Aim of study This work aimed to evaluate the anti-inflammatory effects of a methanolic extract of leaves from A. glandulosa (MEAG), as well as the ethyl acetate fraction (EAFAG) and isolated compound guanidine alkaloid N-1, N-2, N-3-triisopentenylguanidine (AG-1), in experimental in vivo models of inflammation in mice. We also investigated this extract's phenols, flavonoids and flavonol compounds. Materials and methods MEAG (extracted by maceration with methanol), EAFAG (fraction resulting from the partition of the methanolic extract with ethyl acetate) and AG-1 (alkaloid isolated by chromatographic methods) were analysed. MEAG and EAFAG were analysed by HPLC/DAD. The effects of MEAG (30, 100 and 300 mg/kg), EAFAG (30, 100 and 300 mg/kg) and AG-1 (5 and 30 mg/kg) were studied in the following experimental mouse models: paw oedema and myeloperoxidase (MPO) activity, croton-oil-induced ear oedema, leukocyte migration in a pleurisy model induced by carrageenan and zymosan induction of joint inflammation. Results MEAG and EAFAG were analysed by LC/DAD, and phenolic acids (gallic acid and caffeic acid) and flavonoids (myricetin-3-O-α-rhamnopyranoside and quercetin) were detected. MEAG, EAFAG and AG-1 were used in the carrageenan-induced paw oedema model and showed maximum inhibitions of 60.10% (MEAG, 2 h, 300 mg/kg) and 66.21% (EAFAG, 2 h, 300 mg/kg). AG-1 at 5 mg/kg showed significant inhibition, ranging from 60.92% to 63.13%, at all evaluated times, and the 30 mg/kg dose showed inhibition of 42.12% (1 h) and 40.36% (2 h). MEAG (37%, 46.1% and 68.11%) and EAFAG (31%, 42.21% and 48.93%), at doses of 30, 100 and 300 mg/kg, respectively, significantly reduced the increase in MPO activity, and AG-1 (5 and 30 mg/kg) showed inhibition of 64.62% and 65.12%, respectively. In the pleurisy model, MEAG (300 mg/kg), EAFAG (300 mg/kg) and AG-1 (30 mg/kg) significantly reduced the migration of total leukocytes with maximal inhibition of 80.90%, 83.17% and 89.39%, respectively. In the croton oil model, pretreatment with MEAG (0.1, 0.3 and 1 mg/ear) increased the diameter of the right ear (30.32%, 48.87% and 53.09%, respectively). Finally, MEAG (100 and 300 mg/kg; 33.11% and 56.03%) and EAFAG (100 and 300 mg/kg; 36.89% and 50.53%) reduced zymosan-induced oedema formation. Conclusions To the best of our knowledge, these results are the first to demonstrate that A. glandulosa exhibits oral and topical anti-inflammatory activity. This study detected alkaloid and phenol/polyphenolic compounds in A. glandulosa, which may help to explain the ethnobotanical use of this plant in traditional medicine in Brazil to treat immune-mediated inflammatory diseases. |
| Databáze: | OpenAIRE |
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