New perspectives on the cardioprotective phosphonate effect of the spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: an hemodynamic and 31P NMR study in rat hearts
| Autor: | Eziana Maurelli, Thierry Tron, Jean-Louis Gallis, Sylvia Pietri, Marcel Culcasi, Malvina Miollan, Marie-Christine Delmas-Beauvieux |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Cardiac function curve Magnetic Resonance Spectroscopy Oxide Ischemia Hemodynamics Pyrroline In Vitro Techniques Biochemistry Medicinal chemistry Nitrone Cyclic N-Oxides chemistry.chemical_compound Organophosphorus Compounds Nuclear magnetic resonance Physiology (medical) medicine Animals Rats Wistar chemistry.chemical_classification Superoxide Phosphorus Isotopes Heart medicine.disease Phosphonate Rats chemistry Spin Labels |
| Zdroj: | Free Radical Biology and Medicine. 27:34-41 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(99)00033-7 |
| Popis: | The spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) is an improved ESR probe to assess superoxide (O 2 •− ) formation in the postischemic heart. We recently found that DEPMPO pretreatment improves recovery of cardiac function with the concomitant inhibition of postischemic O 2 •− production. By perfusing diethyl methylphosphonate MeP(O)(OEt) 2 to ischemic-reperfused isolated rat hearts, we provide hemodynamic evidence that this preservation, which exerts during ischemia, is in fact specific to the phosphonate group. Using 31 P NMR on intact rat hearts, it was also found that the “phosphonate effect” of DEPMPO is related to the preservation of ATP levels during ischemia, when compared to 5,5-dimethyl-1-pyrroline N-oxide. This mechanism may be a means of reducing the potency of cardiac tissue to produce O 2 •− during reperfusion. |
| Databáze: | OpenAIRE |
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