Evaluation of herpes simplex virus susceptibility to acyclovir using an enzyme-linked immunosorbent assay
| Autor: | C.H. Narbonne, P.Y. Donnio, B. Fauconnier, A. Ruffault, P.M. André |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Simplexvirus
food.ingredient viruses Acyclovir Enzyme-Linked Immunosorbent Assay Microbial Sensitivity Tests Biology medicine.disease_cause Thymidine Kinase Herpesviridae Virus Fixatives food Alphaherpesvirinae medicine Animals Aciclovir Vero Cells Antiserum Dose-Response Relationship Drug General Medicine biology.organism_classification Virology Herpes simplex virus Thymidine kinase medicine.drug |
| Zdroj: | Annales de l'Institut Pasteur / Virologie. 139:185-195 |
| ISSN: | 0769-2617 |
| Popis: | An in situ ELISA was performed directly on the adherent cell monolayer in order to determine the susceptibility of herpes simplex virus isolates to acyclovir. Various fixation procedures and antisera conjugated to different enzymes were tested. The use of glutaraldehyde for fixation and beta-galactosidase as a labelling enzyme was shown to give the best results. As with other currently used assays, 50% inhibitory doses were subject to an inoculum effect. The data obtained indicate that this assay is suitable for routine determination of herpes simplex virus susceptibility to antiviral drugs. |
| Databáze: | OpenAIRE |
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