Screening candidate genes related to volatile synthesis in shiitake mushrooms and construction of regulatory networks to effectively improve mushroom aroma
| Autor: | Chen Wanchao, Di Wu, Jie Feng, Wen Li, Zhong Zhang, Jin-Bin Wang, Jing-Song Zhang, Yan Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Volatile Organic Compounds
Candidate gene Mushroom Nutrition and Dietetics Shiitake Mushrooms food and beverages Biology biology.organism_classification Biosynthetic Pathways Flavoring Agents Fungal Proteins Transcriptome Lentinula Biochemistry Odorants Gene cluster Gene Regulatory Networks KEGG Agronomy and Crop Science Gene Flavor Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 101:5618-5626 |
| ISSN: | 1097-0010 0022-5142 |
| DOI: | 10.1002/jsfa.11213 |
| Popis: | BACKGROUND Metabolite formation is a physiological stress response during the growth and development of shiitake mushrooms (Lentinula edodes). The characteristic flavor metabolites are important quality components in shiitake mushrooms. To investigate the formation mechanisms of characteristic flavor metabolites, transcriptome analyses were performed on shiitake mushrooms harvested at different growth stages. RESULTS In total, 30 genes related to the synthesis of characteristic volatiles of mushrooms were identified via screening. Through KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of the selected genes and correlation analyses of gene expressions, the main volatile synthesis pathways were determined as histidine metabolism, glutathione metabolism and biosynthesis of unsaturated fatty acids. Gene cluster and correlation analyses were performed to clarify the combined effects of different genes in the enzymatic reactions. Further, a correlation network of candidate genes was built based on the gene expression levels. CONCLUSION The activities of flavor synthases and the content of characteristic flavor metabolites were analyzed; the enzyme activity changes and metabolic product distribution sites were clarified. A synthesis and regulation network was constructed for the candidate genes and characteristic volatiles, and information was obtained for 16 hub genes. Moreover, it was essential to identify and characterize the key genes and synthases involved in the synthesis of the characteristic volatiles of mushrooms. This information provides us with a better understanding of the biosynthesis and regulation of the volatiles, which will lay the foundation for improving the quality of shiitake mushrooms. © 2021 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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