Organ-Sparing Microsurgical Resection of Incidental Testicular Tumors Plus Microdissection for Sperm Extraction and Cryopreservation in Azoospermic Patients: Surgical Aspects and Technical Refinements
| Autor: | Kelly S. Athayde, Marcello Cocuzza, Miguel Srougi, Alvaro S. Sarkis, Giovanni Guido Cerri, Jorge Hallak |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Microsurgery endocrine system medicine.medical_specialty Urologic Surgical Procedures Male Urology medicine.medical_treatment Malignancy Urologic Surgical Procedure Spermatic cord Testicular Neoplasms Humans Medicine Microdissection Azoospermia Cryopreservation Frozen section procedure business.industry medicine.disease Testicular sperm extraction Surgery medicine.anatomical_structure Tissue and Organ Harvesting business |
| Zdroj: | Urology. 73:887-891 |
| ISSN: | 0090-4295 |
| DOI: | 10.1016/j.urology.2008.08.510 |
| Popis: | Introduction The management of nonpalpable testicular masses is a challenging task, and coexisting infertility can further complicate the treatment decisions. We present our technique for microsurgical organ-sparing resection of incidental nonpalpable testicular nodules combined with microdissection for testicular sperm extraction and tissue cryopreservation in azoospermic patients. Technical Considerations Five infertile patients with azoospermia presented with nonpalpable hypoechoic testicular masses that were detected by ultrasonography and underwent organ-sparing surgery. The testis was delivered through an inguinal incision, and the blood circulation was interrupted with a vascular clamp placed on the spermatic cord. Sludged ice was used to prevent warm ischemia, and a temperature probe was used to control the temperature at 12°-15°C. Real-time reflex ultrasonography was used to locate the tumor, and a stereotaxic hook-shaped needle was inserted under ultrasound guidance. The needle was placed adjacent to the tumor to guide the microsurgical resection. The tunica albuginea was incised over the tumor, which was dissected and removed, along with the adjoining parenchymal tissue. Frozen section studies were performed and, if malignancy was confirmed, biopsies of the tumor cavity margins and remaining parenchyma were obtained to ensure the absence of residual tumor. Microdissection was performed for excision of selected enlarged tubules that were processed and cryopreserved. Conclusions We present a technique for microsurgical organ-sparing resection of testicular tumor and sperm extraction that can be used in selected infertile patients with azoospermia in whom incidental masses have been diagnosed by ultrasonography. This conservative approach should be especially considered for patients with a solitary testis or bilateral tumors. |
| Databáze: | OpenAIRE |
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