Histological evaluation of skin reconstruction using artificial dermis
| Autor: | Risako Matsui, Mikio Koide, Jun Konishi, Kenichi Osaki, Naganari Okura, Kazuhito Ikegami |
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| Rok vydání: | 1996 |
| Předmět: |
Materials science
Biophysics Bioengineering Matrix (biology) Artificial skin Biomaterials Rats Sprague-Dawley chemistry.chemical_compound Dermis Skin Physiological Phenomena medicine High activity Animals Skin Skin Artificial Thymidine analogue Biomaterial Histology Anatomy Skin Transplantation Rats medicine.anatomical_structure chemistry Mechanics of Materials Evaluation Studies as Topic Ceramics and Composites Wounds and Injuries Cattle Bromodeoxyuridine |
| Zdroj: | Biomaterials. 17(10) |
| ISSN: | 0142-9612 |
| Popis: | An artificial dermis, composed of a collagen matrix, was applied to a full-thickness skin defect prepared on the back of rats. Two weeks later, a thin split-thickness skin autograft was overlaid on the matrix at each recipient site. The dermal layer at the recipient sites was 1.02 mm thick with prior application of artificial dermis, as compared with the 0.46 mm thickness observed without such pretreatment. Histologically, the split-thickness skin graft normally lies with no gap on the artificial dermis, which looks like natural dermis. Six days after grafting, the epithelial basal cells in the grafts showed an active uptake of bromodeoxyuridine (a thymidine analogue), indicating high activity of cell proliferation. About 50 and 20% respectively of the artificial dermis remained at each recipient site at 12 and 20 weeks after its application (after the skin defect). This finding indicates that bovine collagen, which is a constituent of the artificial dermis, is gradually replaced by the host tissue. |
| Databáze: | OpenAIRE |
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