Monoclonal antibodies identify the protective antigens of sporozoites of Plasmodium knowlesi
| Autor: | Alan H. Cochrane, Ruth S. Nussenzweig, Victor Nussenzweig, Robert W. Gwadz, Ferrucio Santoro |
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| Rok vydání: | 1982 |
| Předmět: |
Plasmodium
medicine.drug_class Spleen Antigen-Antibody Complex Monoclonal antibody Serology Mice Antigen parasitic diseases medicine Animals Lymphocytes Antigens Spores Bacterial Infectivity Mice Inbred BALB C Hybridomas Multidisciplinary biology Antibodies Monoclonal biology.organism_classification Macaca mulatta Virology Malaria Molecular Weight Disease Models Animal medicine.anatomical_structure Plasmodium knowlesi Antigens Surface biology.protein Antibody Plasmacytoma Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 79:5651-5655 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.79.18.5651 |
| Popis: | Nine monoclonal antibodies against surface antigens of sporozoites of the simian malaria parasite Plasmodium knowlesi were produced by fusion of plasmacytoma cells with spleen cells of a mouse immunized with the parasites. Immunoprecipitation of extracts of [35S]methionine-labeled sporozoites with seven of the monoclonals identified the same three polypeptides with apparent molecular weights of 52,000 (Pk52), 50,000 (Pk50) and 42,000 (Pk42). These antigens also were recognized by serum of a rhesus monkey immunized with and protected against P. knowlesi sporozoites. Pulse--chase experiments indicated that the higher molecular weight proteins are precursors of Pk42. As shown by trypsin treatment of viable sporozoites, Pk42 is a surface antigen whereas Pk52 and Pk50 appear to be intracellular. Three of the monoclonal antibodies also reacted with a membrane antigen of sporozoites of another simian malaria, P. cynomolgi, and one monoclonal antibody reacted with sporozoites of human malaria, P. falciparum. When assayed for sporozoite neutralizing activity, most of the antibodies and their Fab fragments, which recognize Pk52, Pk50, and Pk42, abolished parasite infectivity. |
| Databáze: | OpenAIRE |
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