The HIV aspartyl protease inhibitor ritonavir impairs planktonic growth, biofilm formation and proteolytic activity inTrichosporonspp
| Autor: | Jonathas Sales de Oliveira, Raimunda Samia Nogueira Brilhante, Patrícia Bruna Leite Mendes, Lucas Pereira de Alencar, Zoilo Pires de Camargo, Rosana Serpa, Ana Raquel Colares de Andrade, Vandbergue Santos Pereira, Rossana de Aguiar Cordeiro, Jônatas da Silva Franco, Débora de Souza Collares Maia Castelo-Branco, Reginaldo Gonçalves de Lima Neto, Marcos Fábio Gadelha Rocha, José Júlio Costa Sidrim, Antonio José de Jesus Evangelista |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antifungal Agents medicine.medical_treatment 030106 microbiology Microbial Sensitivity Tests Trichosporon asahii Aquatic Science Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Trichosporon medicine HIV Protease Inhibitor Drug Interactions Protease inhibitor (pharmacology) Water Science and Technology Ritonavir Protease biology Biofilm Biofilm matrix HIV Protease Inhibitors biochemical phenomena metabolism and nutrition Plankton biology.organism_classification Biofilms Peptide Hydrolases medicine.drug |
| Zdroj: | Biofouling. 33:640-650 |
| ISSN: | 1029-2454 0892-7014 |
| Popis: | This study evaluated the effect of the protease inhibitor ritonavir (RIT) on Trichosporon asahii and Trichosporon inkin. Susceptibility to RIT was assessed by the broth microdilution assay and the effect of RIT on protease activity was evaluated using azoalbumin as substrate. RIT was tested for its anti-biofilm properties and RIT-treated biofilms were assessed regarding protease activity, ultrastructure and matrix composition. In addition, antifungal susceptibility, surface hydrophobicity and biofilm formation were evaluated after pre-incubation of planktonic cells with RIT for 15 days. RIT (200 μg ml−1) inhibited Trichosporon growth. RIT (100 μg ml−1) also reduced protease activity of planktonic and biofilm cells, decreased cell adhesion and biofilm formation, and altered the structure of the biofilm and the protein composition of the biofilm matrix. Pre-incubation with RIT (100 μg ml−1) increased the susceptibility to amphotericin B, and reduced surface hydrophobicity and cell adhesion. These re... |
| Databáze: | OpenAIRE |
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