Role of TEFFECTOR/MEMORY Cells, TBX21 Gene Expression and T-Cell Homing Receptor on Type 1 Reaction in Borderline Lepromatous Leprosy Patients

Autor: Flávio Alves Lara, José Augusto da Costa Nery, Euzenir Nunes Sarno, Danuza Esquenazi, Iris Maria Peixoto Alvim, Luciana Nahar dos Santos, Pedro Henrique Lopes da Silva
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Bacterial Diseases
Antigens
Differentiation
T-Lymphocyte
CD4-Positive T-Lymphocytes
Male
0301 basic medicine
Physiology
Gene Expression
lcsh:Medicine
CD8-Positive T-Lymphocytes
Pathology and Laboratory Medicine
Células T citotóxicas
White Blood Cells
Interleukin 21
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Cytotoxic T cell
lcsh:Science
Immune Response
Membrane Glycoproteins
Multidisciplinary
Expressão genetica
T Cells
GATA3
Borderline lepromatous leprosy
Hematology
Middle Aged
Mycobacterium Leprae
Body Fluids
Up-Regulation
3. Good health
Actinobacteria
Mycobacterium leprae
Infectious Diseases
Blood
medicine.anatomical_structure
Female
Cellular Types
Anatomy
Lesões
Inflamation
Research Article
Neglected Tropical Diseases
Adult
Adolescent
Immune Cells
T cell
Immunology
Receptors
Lymphocyte Homing
Cytotoxic T cells
Biology
Young Adult
03 medical and health sciences
Signs and Symptoms
Antigen
Diagnostic Medicine
Leprosy
Genetics
medicine
Humans
Aged
Inflammation
Blood Cells
Hanseníase
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
Tropical Diseases
medicine.disease
Molecular biology
Inflamação
030104 developmental biology
Lesions
lcsh:Q
Gene expression
T-Box Domain Proteins
Ex vivo
CD8
030215 immunology
Zdroj: PLoS ONE, Vol 11, Iss 10, p e0164543 (2016)
Repositório Institucional da FIOCRUZ (ARCA)
Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
PLoS ONE
ISSN: 1932-6203
Popis: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil / Universidade do Estado do Rio de Janeiro. Escola de Ciências Mèdicas. Departamento de Patologia e Laboratórios. Rio de Janeiro, RJ, Brasil. In spite of hyporesponsivity to Mycobacterium leprae, borderline lepromatous (BL) patients show clinical and immunological instability, and undergo frequent acute inflammatory episodes such as type 1 reaction (T1R), which may cause nerve damages. This work focused on the participation of T cell subsets from blood and skin at T1R onset. We observed a significantly increased ex vivo frequency of both effector and memory CD4+ and CD8+ T cells in T1R group. Besides, ex vivo frequency of T cell homing receptor, the Cutaneous Leukocyte-associated Antigen (CLA) was significantly increased in T cells from T1R patients. M. leprae induced a higher frequency of CD4+ TEM and CD8+ TEF cells, as well as of CD8+/TEMRA (terminally differentiated effector T cells) subset, which expressed high CD69+. The presence of IFN-γ‒producing-CD4+ TEF and naïve and effector CD8+ T lymphocytes was significant in T1R. TBX21 expression was significantly higher in T1R, while BL showed increased GATA3 and FOXP3 expression. In T1R, TBX21 expression was strongly correlated with CD8+/IFN-γ‒ T cells frequency. The number of double positive CD8+/CLA+ and CD45RA+/CLA+ cells was significantly higher in skin lesions from T1R, in comparison with non-reactional BL group. The observed increase of ex vivo T cells at T1R onset suggests intravascular activation at the beginning of reactional episodes. The antigen-specific response in T1R group confirmed the higher number of CD8+/CLA+ and CD45RA+/CLA+ cells in T1R lesions suggests possible migration of these cells activated by M. leprae components inside the vascular compartment to skin and participation in T1R physiopathology.
Databáze: OpenAIRE
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