Ginseng Total Saponin Attenuates Podocyte Apoptosis Induced by Diabetic Conditions Through the Recovery of CD2-Associated Protein
| Autor: | Dong-Soo Ha, Tae-Sun Ha |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Blood Glucose
Glycation End Products Advanced 0301 basic medicine CD2-Associated Protein Blotting Western Panax Medicine (miscellaneous) Apoptosis Podocyte Nephrin Diabetic nephropathy Mice 03 medical and health sciences 0302 clinical medicine Glycation Diabetes Mellitus medicine Animals Diabetic Nephropathies Adaptor Proteins Signal Transducing 030109 nutrition & dietetics Nutrition and Dietetics biology Plant Extracts Podocytes Chemistry Signal transducing adaptor protein Saponins medicine.disease Actins Cell biology Cytoskeletal Proteins medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Signal transduction Phytotherapy Signal Transduction |
| Zdroj: | Journal of Medicinal Food. 22:170-177 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2017.4139 |
| Popis: | CD2-associated protein (CD2AP), an adaptor protein, plays several important roles in podocyte function, linking slit diaphragms to actin-based cytoskeleton and sending survival signals. Here, we investigated whether ginseng total saponin (GTS) had a protective role in the changes of podocyte CD2AP protein and podocyte apoptosis under in vitro diabetic conditions. Conditionally immortalized mouse podocytes cultured with normal glucose (5 mM) or high glucose (30 mM) and with or without advanced glycosylation end products were treated with GTS. We found that CD2AP co-localized with the F-actin fibers in podocyte cytoplasm using confocal imaging; however, diabetic conditions caused the podocytes to diminish and conglomerate CD2AP stainings in the peripheral cytoplasm, which were recovered by GTS. Diabetic conditions also suppressed CD2AP protein levels at 6 and 24 h in western blotting. These phenotypical changes of CD2AP protein were mitigated by GTS. Diabetic conditions also induced podocyte apoptosis at 24 h, which were attenuated by GTS. These findings provide a novel mechanism that diabetic conditions induce quantitative and qualitative changes of podocyte CD2AP protein and apoptosis, which would be restored by GTS. |
| Databáze: | OpenAIRE |
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