Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells
Autor: | Zhiwei He, Guo-Liang Huang, Huahui Li, Bin-Bin Li, Xia Kong |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Gene Expression lcsh:Medicine Antineoplastic Agents Biology Real-Time Polymerase Chain Reaction complex mixtures Catechin 03 medical and health sciences 0302 clinical medicine Cell Line Tumor microRNA Carcinoma medicine Humans heterocyclic compounds Nasopharyngeal Carcinoma Epigallocatechin-3-gallate lcsh:R Antitumor Agents Computational Biology food and beverages Nasopharyngeal Neoplasms General Medicine Gallate medicine.disease MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Nasopharyngeal carcinoma Cell culture 030220 oncology & carcinogenesis Immunology Cancer research Original Article sense organs Function (biology) Signal Transduction |
Zdroj: | Chinese Medical Journal, Vol 130, Iss 1, Pp 93-99 (2017) Chinese Medical Journal |
ISSN: | 0366-6999 |
Popis: | Background: Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma (NPC), but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs (miRNAs) in NPC cells. Methods: Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis. Results: A total of 14 miRNAs exhibited >2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs. Conclusion: EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG. |
Databáze: | OpenAIRE |
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