Glycophenotypic alterations induced by Pteridium aquilinum in mice gastric mucosa: synergistic effect with Helicobacter pylori infection
| Autor: | Steven R. Head, Ana Magalhães, Suzanne Papp, Joana Gomes, Valérie Michel, Celso A. Reis, Ana Sofia Carvalho, Gilberto E. Hernandez, Fátima Gärtner, Eliette Touati, Leonor David |
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| Přispěvatelé: | Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Universidade do Porto, The SCRIPPS Research Institute (SCRIPPS), University of California [Los Angeles] (UCLA), University of California-University of California, Pathogenèse de Helicobacter, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by Fundação para a Ciência e a Tecnologia – [FCT (PIC/IC/82716/2007), grant number SFRH/BD/40563/2007 to J.G and SFRH/BPD/75871/2011 to A.M.], and the Programa de Acções Universitárias Integradas Luso-Francesas - PAULIF [grant number LC/HS/ED/2010-308, F-TC04/11]. The resources and collaborative efforts provided by the Consortium for Functional Glycomics were funded by grant NIGMS-GM62116., Universidade do Porto = University of Porto, The Scripps Research Institute [La Jolla, San Diego], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Bacterial Diseases
Mouse MESH: Carbohydrate Metabolism MESH: Helicobacter Infections/pathology Glycobiology Biochemistry Transcriptome Mice 0302 clinical medicine [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Gastric Mucosa/drug effects MESH: Animals Histochemistry MESH: Helicobacter Infections/complications 0303 health sciences Cocarcinogenesis Multidisciplinary biology Enzyme Classes Immunochemistry Cancer Risk Factors Environmental Causes of Cancer Animal Models Immunohistochemistry MESH: Helicobacter pylori/isolation & purification Enzymes 3. Good health Infectious Diseases Phenotype medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cytochemistry Carbohydrate Metabolism Medicine medicine.symptom Immunocytochemistry C1GALT1 Research Article MESH: Plant Extracts/toxicity MESH: Pteridium/chemistry Science Carbohydrates Inflammation [SDV.CAN]Life Sciences [q-bio]/Cancer MESH: Stomach Neoplasms/complications MESH: Phenotype Helicobacter Infections Microbiology 03 medical and health sciences Model Organisms Stomach Neoplasms MESH: Cocarcinogenesis [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Glycosyltransferase Gastric mucosa medicine Animals Biology MESH: Mice Carcinogen MESH: Helicobacter Infections/microbiology Glycoproteins Pteridium 030304 developmental biology Helicobacter pylori Plant Extracts Glycosyltransferases MESH: Immunohistochemistry biology.organism_classification Gastric Mucosa biology.protein Pteridium aquilinum MESH: Stomach Neoplasms/etiology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (6), pp.e38353. ⟨10.1371/journal.pone.0038353⟩ PLoS ONE, Vol 7, Iss 6, p e38353 (2012) PLoS ONE, 2012, 7 (6), pp.e38353. ⟨10.1371/journal.pone.0038353⟩ |
| ISSN: | 1932-6203 |
| Popis: | International audience; The bracken fern Pteridium aquilinum is a plant known to be carcinogenic to animals. Epidemiological studies have shown an association between bracken fern exposure and gastric cancer development in humans. The biological effects of exposure to this plant within the gastric carcinogenesis process are not fully understood. In the present work, effects in the gastric mucosa of mice treated with Pteridium aquilinum were evaluated, as well as molecular mechanisms underlying the synergistic role with Helicobacter pylori infection. Our results showed that exposure to Pteridium aquilinum induces histomorphological modifications including increased expression of acidic glycoconjugates in the gastric mucosa. The transcriptome analysis of gastric mucosa showed that upon exposure to Pteridium aquilinum several glycosyltransferase genes were differently expressed, including Galntl4, C1galt1 and St3gal2, that are mainly involved in the biosynthesis of simple mucin-type carbohydrate antigens. Concomitant treatment with Pteridium aquilinum and infection with Helicobacter pylori also resulted in differently expressed glycosyltransferase genes underlying the biosynthesis of terminal sialylated Lewis antigens, including Sialyl-Lewis x. These results disclose the molecular basis for the altered pattern of glycan structures observed in the mice gastric mucosa. The gene transcription alterations and the induced glycophenotypic changes observed in the gastric mucosa contribute for the understanding of the molecular mechanisms underlying the role of Pteridium aquilinum in the gastric carcinogenesis process. |
| Databáze: | OpenAIRE |
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