Argemone oil, an edible oil adulterant, induces systemic immunosuppression in Balb/c mice in an oral 28 days repeated dose toxicity study
| Autor: | Sachin Kumar, Mukul Das, Rajnish Kumar Chaturvedi, Anjaneya Ayanur, Sarika Yadav, Prachi Tewari, Payal Mandal, Anurag Tripathi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty T-Lymphocytes medicine.medical_treatment Lymphocyte Administration Oral Nitric Oxide Synthase Type II Spleen Thymus Gland Toxicology BALB/c Mice 03 medical and health sciences Immune system Antigen Internal medicine Immune Tolerance medicine Animals Plant Oils Cell Proliferation B-Lymphocytes Mice Inbred BALB C biology Tumor Necrosis Factor-alpha Chemistry Interleukins Organ Size General Medicine Epidemic dropsy Flow Cytometry biology.organism_classification Mixed lymphocyte reaction medicine.disease Intestines 030104 developmental biology medicine.anatomical_structure Cytokine Endocrinology Cyclooxygenase 2 Female Antigens CD1d |
| Zdroj: | Chemico-Biological Interactions. 287:57-69 |
| ISSN: | 0009-2797 |
| Popis: | Consumption of edible oils contaminated with Argemone oil (AO) leads to a clinical condition called “Epidemic dropsy”. Earlier studies have reported that metabolism and oxidative stress primarily contributes to AO toxicity, however, the involvement of immune system has not been assessed so far. Therefore, the present study was undertaken to systematically assess the effect of AO exposure on the function of immune system in Balb/c mice. The repeated exposure of AO for 28 days caused prominent regression of spleen and thymus; severe inflammatory changes in spleen depicted by the loss of distinct follicles, increased megakaryocyte infiltration, and enhanced expression levels of inflammatory markers (iNOS & COX-2). At the functional level, AO exposure significantly abrogated the mixed lymphocyte reaction and mitogen-stimulated lymphoproliferative activity of T and B cells, which is reflective of profound lymphocyte dysfunction upon antigen exposure. In concordance with the loss in functional activity of lymphocytes in AO exposed animals, it was found the AO altered the relative percentage of CD3+, CD4+, and CD28 + T cells. Further, there was a marked decrease in the relative distribution of cells with prominent MHC I and CD1d expression in AO exposed splenocytes. Moreover, reduced levels of immune stimulatory cytokines (TNF-α, IFN-γ, IL-2, IL-4, and IL-6), and increased levels of immunosuppressive cytokine IL-10 were detected in the serum of AO treated mice. Along with T and B cells, AO exposure also affected the phenotype and activation status of macrophages suggesting the inclination towards “alternative activation of macrophages”. Altogether, these functional changes in the immune cells are contributing factors in AO induced immunosuppression. |
| Databáze: | OpenAIRE |
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