Isolation and partial characterization of the neutral glycosphingolipids and gangliosides of the human heart
| Autor: | Spyridon D. Moulopoulos, J. N. Karli, Gabriel M. Levis |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Adult
Ceramide Ganglioside Chromatography Chromatography Gas Globoside Globosides Clinical chemistry Myocardium Organic Chemistry Cell Biology Biochemistry Thin-layer chromatography Glycosphingolipids Sialic acid chemistry.chemical_compound chemistry Cerebrosides Gangliosides Humans lipids (amino acids peptides and proteins) Gas chromatography Lipidology |
| Zdroj: | Lipids. 14(1) |
| ISSN: | 0024-4201 |
| Popis: | The glycosphingolipids (GLS) of the human heart muscle have been isolated from total lipids by column and thin layer chromatography and their sugars and fatty acids analyzed by gas liquid chromatography. Hearts from traffic victims were obtained at autopsy between 12 and 16 hr after death and dissected into parts (left and right ventricular walls, intraventricular septum and papillary muscle). The neutral GSL content for those parts of the hearts of two males aged 22 and one female aged 14 ranged from about 90 to 160 nmoles/g wet weight. Trihexosyl ceramide and globoside were the most abundant neutral GSL. Total ganglioside content was about 50 nmoles/g wet weight, and the most abundant gangliosides were partially characterized as GM3 and GM1; other mono-, di- and trisialogangliosides were also present. Differences in the content and composition of neutral GSL and gangliosides between the heart and other human tissues are discussed. It is concluded that the patterns of these two GSL fractions of the heart are more complex than those of most of the extraneural human tissues. |
| Databáze: | OpenAIRE |
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