B cell activation in the cecal patches during the development of an experimental colitis model
| Autor: | Akinori Hosoya, Hajime Karasuyama, Takashi Nagaishi, Takahiro Adachi, Tetsuya Nakamura, Yudai Kojima, Nisha Jose, Michio Onizawa, Mamoru Watanabe, Shigeru Oshima, Taro Watabe, Naoya Tsugawa, Yasuhiro Nemoto |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Intravital Microscopy Colon Biophysics Appendix Lymphocyte Activation Biochemistry Pathogenesis Oxazolone 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Immune system Follicular phase Medicine Animals Humans Calcium Signaling Colitis Molecular Biology Cecum B-Lymphocytes business.industry Experimental colitis Cell Biology Intravital Imaging medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure Tertiary Lymphoid Structures chemistry business 030217 neurology & neurosurgery |
| Zdroj: | Biochemical and biophysical research communications. 496(2) |
| ISSN: | 1090-2104 |
| Popis: | Although previous studies have suggested that appendix seems to be involved in the colitis, the role of this in the pathogenesis remains unclear. In this study, we assessed the importance of appendiceal lymphoid follicles, specifically the cecal patches (CP) in mice, using an experimental colitis model. Treatment with oxazolone resulted in ulcerations particularly at CP with follicular expansion as well as colitis. The colitis was attenuated by either appendectomy or the absence of mature B cells. We therefore established an intravital imaging system accompanied by the fluorescence resonance energy transfer technology to analyze the dynamic immune response of CP B cells. Our observation revealed frequent Ca2+ signaling in CP B cells during the early phase of colitis development. These findings suggested that the CP B cells may be involved in the pathogenesis of colitis including inflammatory bowel diseases in humans. |
| Databáze: | OpenAIRE |
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