Quantification of picogram levels of specific DNA immobilized in microtiter wells
| Autor: | Kazuo Takemura, Osamu Kosuda, Hiroshi Yokota, Yoshiho Nagata, Tetsuya Kikuchi, Keiko Yokoo |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Biophysics
Biotin Avidin-biotinylated β-galactosidase complex Microtiter well Biochemistry Styloviridae chemistry.chemical_compound Structural Biology Immobilized DNA Genetics DNA probe quantitation Molecular Biology Chromatography Chemistry Nowadioisotopic DNA probe Microchemistry Hybridization probe Nucleic Acid Hybridization Cell Biology Avidin beta-Galactosidase Bacteriophage lambda Fluorescence Molecular hybridization Biotinylation DNA Viral DNA |
| Zdroj: | FEBS Letters. (2):379-382 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(85)80814-0 |
| Popis: | A non-radioisotopic and sensitive method for quantification of specific DNA immobilized in microtiter wells has been developed. This method is based upon the immobilization of DNA in microtiter wells and hybridization with biotinylated DNA probe which is followed by complexing with avidin-β-galactosidase. By measuring fluorescence emitted from the hydrolyzed product by β-galactosidase of 4-methylumbelliferyl-β-D-galactoside, it has become possible to quantify a few picograms of specific DNA in DNA samples immobilized in plastic microtiter wells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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