Regulated Expression Allows High Level Production and Secretion of HIV-1 gp120 Envelope Glycoprotein in Drosophila Schneider Cells
| Autor: | Anne Delers, Jeffery S. Culp, Brian D. Hellmig, Martin Rosenberg, Thomas J. Matthews, Jeffery A. Beck, Hanne Johansen |
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| Rok vydání: | 1991 |
| Předmět: |
CD4-Positive T-Lymphocytes
Glycosylation medicine.drug_class viruses Molecular Sequence Data Cell Biomedical Engineering Bioengineering HIV Envelope Protein gp120 Biology Monoclonal antibody Applied Microbiology and Biotechnology Cell Line law.invention Viral envelope law medicine Animals Humans Secretion Amino Acid Sequence Cloning Molecular chemistry.chemical_classification Syncytium Antibodies Monoclonal virus diseases Virology Recombinant Proteins Cell biology medicine.anatomical_structure Gene Expression Regulation chemistry Cell culture CD4 Antigens HIV-1 Recombinant DNA Molecular Medicine Drosophila Glycoprotein Plasmids Biotechnology |
| Zdroj: | Nature Biotechnology. 9:173-177 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt0291-173 |
| Popis: | We have established a stable, continuous culture Drosophila Schneider 2 cell line that efficiently expresses a secreted, truncated form of the HIV envelope gp120 protein in a regulated manner. The Drosophila produced recombinant gp120 protein is highly glycosylated, is recognized by gp120-specific monoclonal antibodies, binds to the CD4 receptor and has the ability to inhibit syncytia formation between uninfected CD4+ cells and HIV infected cells. We conclude that this recombinant Drosophila envelope protein is an appropriate mimic of the authentic viral envelope protein. Thus, the Drosophila cell provides a continuous, stable culture system for the efficient expression of secreted forms of complex surface glycoproteins in quantities sufficient for detailed analyses. |
| Databáze: | OpenAIRE |
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