Antibacterial Activity of the Iranian Scorpion’s Crude Venom (Odontobuthus bidentatus) on Gram-positive and Gram-negative Bacteria
| Autor: | Ali Bidmeshkipour, Hani Keshavarz Alikhani, Mohammad Hosseinpour, Jamil Zargan, Abbas Hajizadeh, Ahmad Heydari, Ashkan Haji Nour Mohammadi |
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| Rok vydání: | 2020 |
| Předmět: |
antimicrobial activity
Gram-negative bacteria biology Chemistry Health Toxicology and Mutagenesis gram-positive and gram–negative bacteria Venom Bacillus subtilis Toxicology medicine.disease_cause biology.organism_classification complex mixtures e. coli Microbiology Minimum inhibitory concentration odontobuthus bidentatus lcsh:RA1190-1270 Staphylococcus aureus Escherichia medicine scorpion venom Antibacterial activity s. aureus and b. subtilis Bacteria lcsh:Toxicology. Poisons |
| Zdroj: | Iranian Journal of Toxicology, Vol 14, Iss 2, Pp 105-110 (2020) |
| ISSN: | 2251-9459 2008-2967 |
| DOI: | 10.32598/ijt.14.2.627 |
| Popis: | Background: The venoms of some scorpions are rich in bioactive components that may be used in the development and discovery of new antibacterial drugs. The venoms have many components, such as neurotoxins, salts, proteins and peptides with therapeutic properties, and can rapidly eliminate a broad range of bacteria. This study evaluated the anti-bacterial activity of Odontobuthus bidentatus’ crude venom against typical Gram-positive and negative bacteria, such as Staphylococcus aureus, Bacillus subtilis, and Escherichia coli. Methods: The antibacterial effects of the crude venom were evaluated using Minimal Inhibitory Concentration (MIC) and MTT assays and its IC50 value was determined, using GraphPad software. Results: The crude venom significantly inhibited the growth of both Gram-positive and negative bacteria. Also the MTT results showed that the crude venom significantly reduced the viability of E. Coli, S. Aureus, and B. Subtilis bacteria compared with that for the controls. The IC50 values of the venom for E.coli, S.aureus, and B.subtilis were 30.19, 17.64, and 24.13 µM, respectively. Conclusion: The findings suggest that the venom of O. bidentatus scorpion has antibacterial properties. Also, our results offer preliminary clues toward the development of new antibacterial agents and new drugs with high therapeutic potentials for use in animals and humans. |
| Databáze: | OpenAIRE |
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