The MicroRNA Prediction Models as Ancillary Diagnosis Biomarkers for Urothelial Carcinoma in Patients With Chronic Kidney Disease
| Autor: | An-Lun Li, Che-Yi Chou, Chien-Lung Chen, Kun-Lin Wu, Shih-Chieh Lin, Chi-Ching Chen, Kuo-Hsiung Shu, Hung-Chun Chen, Ming-Chang Wang, Chia-Chu Chang, Bang-Gee Hsu, Mai-Szu Wu, Chiu-Ching Huang, Nianhan Ma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Medicine (General) Bladder cancer medicine.diagnostic_test business.industry Urinary system Urology biofluid Urine Cystoscopy General Medicine Nomogram medicine.disease microRNA (miRNA) R5-920 medicine Medicine Biomarker (medicine) biomarker urothelial carcinoma (UC) business chronic kidney disease Original Research Urine cytology Kidney disease |
| Zdroj: | Frontiers in Medicine, Vol 8 (2021) Frontiers in Medicine |
| ISSN: | 2296-858X |
| DOI: | 10.3389/fmed.2021.726214 |
| Popis: | Background: Urothelial carcinoma is a common urological cancer in patients with chronic kidney disease in Taiwan. However, urinary cystoscopy is an invasive procedure and urine cytology showed low sensitivity for low-grade tumor. High accuracy with noninvasive diagnostic tools for UC is needed for patients with CKD. Methods: A total of 272 urine and 138 plasma samples to detect the miRNA expression levels for establishing UC signatures from patients with CKD. 17 candidate miRNAs of urine and plasma were selected by the PCR array, and a single qRT-PCR assay was used to confirm the results. The website of a smRNA-seq analysis of the clinical specimens was compared to survival status. The miRNA prediction models were produced for predicting UC by multiple logistic regression.Results: Urinary miR-1274a and miR-30a-5p expression levels were significantly lower but miR-19a-5p expression levels were higher in UC when compared with CKD. In plasma samples, miR-155-5p, miR-19b-1-5p, miR-378 and miR-636 showed significantly lower expression in UC compared to those with CKD. The Kaplan-Meier curve showed that lower expression of miR-19a, miR-19b, miR-636 and miR-378, and higher expression of miR-708-5p were associated with poor prognosis in patients with bladder cancer. In addition, we produced classifiers for predicting UC by multiple logistic regression. The urine signature was developed with four miRNAs, and the AUC was 0.8211. Eight miRNA expression levels from both urine and plasma samples were examined, and the AUC was 0.8595.Conclusion: Two miRNA classifiers could improve the drawbacks of current UC biomarker screenings for patients with CKD. |
| Databáze: | OpenAIRE |
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