Beneficial effects of C-peptide on renal morphology in diabetic rats
| Autor: | Xin Gao, Qi-Chang Xia, Da-Fu Cui, Wei Sun, Xiaolong Zhao |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Collagen Type IV
medicine.medical_specialty Time Factors medicine.medical_treatment Biophysics Enzyme-Linked Immunosorbent Assay Biochemistry Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley chemistry.chemical_compound Type IV collagen Internal medicine Diabetes mellitus medicine Animals Diabetic Nephropathies Glycemic Kidney C-Peptide Mesangial cell Reverse Transcriptase Polymerase Chain Reaction Chemistry C-peptide Insulin General Medicine Streptozotocin medicine.disease Glomerular Mesangium Rats Endocrinology medicine.anatomical_structure Gene Expression Regulation Matrix Metalloproteinase 2 medicine.drug |
| Zdroj: | Acta Biochimica et Biophysica Sinica. 42:893-899 |
| ISSN: | 1672-9145 |
| DOI: | 10.1093/abbs/gmq100 |
| Popis: | The study was undertaken to examine the effects of C-peptide on glomerular volume (V(GLOM)), mesangial matrix synthesis, and degradation in streptozotocin (STZ)-diabetic rats with poor or moderate glycemic control. Series 1 (poor glycemic control) included groups of healthy rats, hyperglycemic rats, diabetic insulin-treated rats and diabetic C-peptide-treated rats. Series 2 (moderate glycemic control) included groups of healthy rats, diabetic insulin-treated rats, diabetic insulin- and C-peptide-treated rats. After 8 weeks, the left kidney was excised for evaluation of V(GLOM) and mesangial matrix area via light microscopy. Mesangial cells were cultured for 48 h and type IV collagen expression and matrix metalloproteinase (MMP)-2 expression were measured by ELISA and RT-PCR. The results indicated that in Series 1, C-peptide administration suppressed the diabetes-induced increase in the V(GLOM) and the mesangial matrix area. In Series 2, C-peptide administration resulted in a similar decrease in the V(GLOM) and a greater decrease in the mesangial matrix area when compared with insulin therapy alone. Moreover, C-peptide (300 nM) completely inhibited the glucose-induced increase of the collagen IV mRNA expression and protein concentration in mesangial cells cultured in 30 mM glucose medium. MMP-2 mRNA expression was not influenced by C-peptide. In conclusion, C-peptide administration to STZ-diabetic rats for 8 weeks results in the inhibition of diabetes-induced expansion of the mesangial matrix. This effect is independent of the level of glycemic control and results from the inhibition of diabetes-induced excessive formation of mesangial type IV collagen. |
| Databáze: | OpenAIRE |
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