Enzymatic Oxidation of Butane to 2‐Butanol in a Bubble Column
| Autor: | Frederic Perz, Paul Bubenheim, Miguel Alcalde, Roland Ulber, Andreas Liese, Sebastian Bormann, Dirk Holtmann, Frank Hollmann |
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| Rok vydání: | 2020 |
| Předmět: |
010402 general chemistry
01 natural sciences butanol Catalysis hydroxylation Inorganic Chemistry Hydroxylation chemistry.chemical_compound ddc:570 peroxygenase Organic chemistry Physical and Theoretical Chemistry Hydrogen peroxide 010405 organic chemistry Chemistry Butanol Biowissenschaften Biologie [570] Organic Chemistry Butanone Substrate (chemistry) Butane 0104 chemical sciences Turnover number butane bubble column Bubble column reactor |
| Zdroj: | ChemCatChem 14 (12): 3666-3669 (2020) ChemCatChem, 12(14) |
| ISSN: | 1867-3899 1867-3880 |
| DOI: | 10.1002/cctc.202000431 |
| Popis: | Unspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non-activated C−H bonds using only hydrogen peroxide as a co-substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we demonstrate the application of a peroxygenase for the hydroxylation of the inert, gaseous substrate butane to 2-butanol in a bubble column reactor. The influence of hydrogen peroxide feed rate and enzyme loading on product formation, overoxidation to butanone and catalytic efficiency is investigated at 200 mL scale. The process is scaled up to 2 L and coupled with continuous extraction. This setup allowed the production of 115 mmol 2-butanol and 70 mmol butanone with an overall total turnover number (TTN) of over 15.000, thereby demonstrating the applicability of peroxygenases for preparative hydroxylation of such inert, gaseous substrates at mild reaction conditions. |
| Databáze: | OpenAIRE |
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