Adipose Triglyceride Lipase (ATGL) and Hormone-Sensitive Lipase (HSL) Deficiencies Affect Expression of Lipolytic Activities in Mouse Adipose Tissues
| Autor: | Maria Morak, Albin Hermetter, Guenter Haemmerle, Rudolf Zechner, Gernot Riesenhuber, Manfred Kollroser, Hannes Schmidinger, Gerald N. Rechberger, Florian Kronenberg |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Lipolysis Adipose tissue Hormone-sensitive lipase White adipose tissue Biology Biochemistry Gene Expression Regulation Enzymologic Carboxylesterase Analytical Chemistry Mice Internal medicine Brown adipose tissue medicine Animals Lipase Molecular Biology Triglycerides Mice Knockout Lipoprotein lipase Research food and beverages Sterol Esterase Endocrinology medicine.anatomical_structure Adipose Tissue Adipose triglyceride lipase biology.protein |
| Zdroj: | Molecular & Cellular Proteomics. 11:1777-1789 |
| ISSN: | 1535-9476 |
| Popis: | Adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) are key enzymes involved in intracellular degradation of triacylglycerols. It was the aim of this study to elucidate how the deficiency in one of these proteins affects the residual lipolytic proteome in adipose tissue. For this purpose, we compared the lipase patterns of brown and white adipose tissue from ATGL (-/-) and HSL (-/-) mice using differential activity-based gel electrophoresis. This method is based on activity-recognition probes possessing the same substrate analogous structure but carrying different fluorophores for specific detection of the enzyme patterns of two different tissues in one electrophoresis gel. We found that ATGL-deficiency in brown adipose tissue had a profound effect on the expression levels of other lipolytic and esterolytic enzymes in this tissue, whereas HSL-deficiency hardly showed any effect in brown adipose tissue. Neither ATGL- nor HSL-deficiency greatly influenced the lipase patterns in white adipose tissue. Enzyme activities of mouse tissues on acylglycerol substrates were analyzed as well, showing that ATGL-and HSL-deficiencies can be compensated for at least in part by other enzymes. The proteins that responded to ATGL-deficiency in brown adipose tissue were overexpressed and their activities on acylglycerols were analyzed. Among these enzymes, Es1, Es10, and Es31-like represent lipase candidates as they catalyze the hydrolysis of long-chain acylglycerols. |
| Databáze: | OpenAIRE |
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