Inhibition of the Glycogen Synthase Kinase 3 Family by the Bikinin Alleviates the Long-Term Effects of Salinity in Barley
| Autor: | Mateusz Przyborowski, Jolanta Groszyk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Salinity
Organic Chemistry Aminopyridines Hordeum Succinates General Medicine Catalysis Computer Science Applications Inorganic Chemistry Glycogen Synthase Kinase 3 Plant Breeding Gene Expression Regulation Plant 24-epibrassinolide brassinosteroids BR signaling pathway BZR1 Golden Promise GSK3 Haruna Nijo inhibitor PSII RNA-Seq Brassinosteroids Physical and Theoretical Chemistry Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11644 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms231911644 |
| Popis: | Crops grown under stress conditions show restricted growth and, eventually, reduced yield. Among others, brassinosteroids (BRs) mitigate the effects of stress and improve plant growth. We used two barley cultivars with differing sensitivities to BRs, as determined by the lamina joint inclination test. Barley plants with the 2nd unfolded leaf were sprayed with a diluted series of bikinin, an inhibitor of the Glycogen Synthase Kinase 3 (GSK3) family, which controls the BR signaling pathway. Barley was grown under salt stress conditions up to the start of the 5th leaf growth stage. The phenotypical, molecular, and physiological changes were determined. Our results indicate that the salt tolerance of barley depends on its sensitivity to BRs. We confirmed that barley treatment with bikinin reduced the level of the phosphorylated form of HvBZR1, the activity of which is regulated by GSK3. The use of two barley varieties with different responses to salinity led to the identification of the role of BR signaling in photosynthesis activity. These results suggest that salinity reduces the expression of the genes controlling the BR signaling pathway. Moreover, the results also suggest that the functional analysis of the GSK3 family in stress responses can be a tool for plant breeding in order to improve crops’ resistance to salinity or to other stresses. |
| Databáze: | OpenAIRE |
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