Electrochemical immunosensor based on hydrophilic polydopamine-coated prussian blue-mesoporous carbon for the rapid screening of 3-bromobiphenyl
| Autor: | Zihong Sun, Shidong Fei, Zhigang Luo, Yingju Liu, Hongtao Lei, Cuifen Gan |
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| Rok vydání: | 2014 |
| Předmět: |
Indoles
Materials science Polymers Biomedical Engineering Biophysics chemistry.chemical_element Infrared spectroscopy Nanotechnology Biosensing Techniques Electrochemistry chemistry.chemical_compound Limit of Detection Immunoassay Detection limit Prussian blue Nanocomposite Nanotubes Carbon Biphenyl Compounds Substrate (chemistry) Electrochemical Techniques Equipment Design General Medicine Linear range chemistry Chemical engineering Antibodies Immobilized Porosity Carbon Ferrocyanides Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 59:99-105 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2014.03.034 |
| Popis: | A sensitive electrochemical immunosensor for 3-bromobiphenyl (3-BBP) detection was constructed by employing a new polydopamine coated prussian blue-mesoporous carbon (PDOP/PB/CMK-3) nanocomposite as the substrate platform and multi-horseradish peroxidase-double helix carbon nanotubes-secondary antibody (multi-HRP-DHCNTs-Ab2) as the signal label. PB/CMK-3 was firstly successfully in-situ synthesized with the aid of the CMK-3 reduction, which was characterized by transmission electron microscope (TEM), infrared spectroscopy (IR), X-ray diffraction (XRD) and N2 adsorption–desorption analysis. By using PDOP/PB/CMK-3 as the substrate, it can effectively enhance the specific surface for antigen loading due to the three-dimensional structure of the nanocomposites, while large amount of PB that fixed inside or outside the pore of CMK-3 successfully improved the electrochemical response and the PDOP film can provide a biocompatible environment to maintain the activity of antigen availability. Under the optimized conditions, the proposed immunosensor shows a good current response to 3-BBP in a linear range from 5 pM to 2 nM with a detection limit of 2.25 pM. In addition, the specificity, reproducibility and stability of the immunosensor were also proved to be acceptable, indicating its potential application in environmental monitoring. |
| Databáze: | OpenAIRE |
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