Schistosoma mansoni PCR(+)‐infected individuals in the Sudan present elevated systemic levels of chemokines when compared to uninfected and egg(+) cohorts
| Autor: | Anna Wiszniewsky, Laura E. Layland, Ibtisam A. Goreish, Manuel Ritter, Tayseer Elamin Mohamed Elfaki, Gnatoulma Katawa, N Jelden, Achim Hoerauf, Mike Doenhoff, M E Y A Atti El Mekki, Kathrin Arndts |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Chemokine Adolescent Eggs Immunology Prevalence Enzyme-Linked Immunosorbent Assay Biology Polymerase Chain Reaction law.invention Cohort Studies Sudan 03 medical and health sciences Young Adult 0302 clinical medicine Blood serum Immune system law parasitic diseases Immunology and Allergy Helminths Animals Humans Child Polymerase chain reaction Aged Retrospective Studies Aged 80 and over Original Articles Schistosoma mansoni Middle Aged biology.organism_classification Schistosomiasis mansoni Up-Regulation 030104 developmental biology Child Preschool biology.protein Female Antibody Chemokines 030215 immunology |
| Zdroj: | Clin Exp Immunol |
| Popis: | Summary Infections with Schistosoma mansoni remain a major health problem in the Sudan where endemic communities, such as those in Kassala and Khartoum states, continue to face severe social–economic difficulties. Our previous immunoepidemiological findings revealed different immune [cytokine and S. mansoni egg (SEA) antibody] profiles in individuals with active infections (eggs in stool n = 110), individuals positive for S. mansoni via polymerase chain reaction (PCR) using sera (SmPCR+ n = 63) and those uninfected (Sm uninf). As antibody responses to eggs and worms are known to change during infection, we have expanded the profiling further by determining levels of adult worm (SWA) antibodies and nine chemokines in the serum of each individual in the three different cohorts. With the exception of C-C motif chemokine ligand (CCL)2, all measured chemokines were significantly higher in SmPCR+ individuals when compared to the egg+ group and in addition they also presented elevated levels of SWA-specific immunoglobulin (Ig)G2. Multivariable regression analysis further revealed that infection per se was strongly linked to SWA-specific IgG3 levels and CCL5 was strongly associated with a SmPCR+ diagnostic state. In the absence of PCR diagnostics that recognize juvenile worms or schistosomulae motives, identifying schistosome-specific traits should provide better insights into current prevalence rates in endemic communities and, in doing so, take into consideration PCR+ non-egg+ individuals in current treatment programmes. |
| Databáze: | OpenAIRE |
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