Effects of Losartan, HO‐1 Inducers or HO‐1 Inhibitors on Erectile Signaling in Diabetic Rats
| Autor: | Soheir Mahfouz, Mohamed T. Abdel Aziz, Hazem Atta, Dina Sabry, Hanan Fouad, Ahmed B. Demery, Taymour Mostafa, Amira M. Senbel, Mohamed Farid El Asmer, Amira Hassouna, Ahmed T. Abdel Aziz, Laila A. Rashed |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Urology Endocrinology Diabetes and Metabolism Gene Expression Pharmacology Losartan Diabetes Mellitus Experimental chemistry.chemical_compound Endocrinology Erectile Dysfunction Internal medicine Diabetes mellitus medicine Animals Cyclic guanosine monophosphate Antihypertensive Agents Reverse Transcriptase Polymerase Chain Reaction business.industry Penile Erection Intracellular Signaling Peptides and Proteins medicine.disease Receptor antagonist Angiotensin II COPP Rats Heme oxygenase Disease Models Animal Psychiatry and Mental health Treatment Outcome medicine.anatomical_structure Reproductive Medicine chemistry Cavernous tissue RNA Carrier Proteins business Heme Oxygenase-1 medicine.drug |
| Zdroj: | The Journal of Sexual Medicine. 6:3254-3264 |
| ISSN: | 1743-6095 |
| Popis: | Introduction Activation of the renin‐angiotensin system which is common in diabetes mellitus might affect heme oxygenase (HO‐1) gene expression. Aim Assessment of the effects of administration of angiotensin II (Ang II) receptor antagonist (losartan) with HO‐1 inducer or inhibitor on erectile signaling in diabetic rats. Materials and Methods Seventy male rats were divided equally into seven groups; healthy controls, streptozotocin‐induced diabetic rats, rats on citrate buffer, diabetic rats on losartan, diabetic rats on HO‐1 inducer (cobalt protoporphyrin [CoPP]), diabetic rats on losartan and CoPP, and diabetic rats on losartan and HO‐1 inhibitor (stannus mesoporphyrin [SnMP]). Main Outcome Measure HO enzyme activity, HO‐1 gene expression, cyclic guanosine monophosphate (cGMP) assay, intracavernosal pressure (ICP), and cavernous tissue sinusoids surface area. Results HO‐1 gene expression, HO enzymatic activity, and cGMP were significantly decreased in the cavernous tissue of diabetic rats. These parameters were significantly elevated with the use of CoPP that restored the normal control levels of HO enzyme activity. Administration of losartan exhibited a significant enhancing effect on these parameters compared with the diabetic group, but not restored to the control levels, whereas administration of CoPP combined with losartan led to the restoration of their normal levels. ICP demonstrated significant decline in diabetic rats. The use of CoPP and/or losartan led to its significant improvement compared with diabetic rats. Administration of either losartan and/or CoPP led to a significant increase in the cavernous sinusoids surface area of diabetic rats. Administration of losartan with SnMP significantly decreased the enhancing effect of losartan on the studied parameters. Conclusion The decline in erectile function in diabetes mellitus could be attributed to the downregulation of HO‐1 gene expression. HO‐1 induction added to Ang II receptor antagonist could improve erectile function. Abdel Aziz MT, El Asmer MF, Mostafa T, Atta H, Mahfouz S, Fouad H, Rashed L, Sabry D, Hassouna A, Abdel Aziz AT, Senbel A, and Demery A. Effects of losartan, HO‐1 inducers or HO‐1 inhibitors on erectile signaling in diabetic rats. |
| Databáze: | OpenAIRE |
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