Absence of Borrelia burgdorferi in the myocardium of subjects with normal left ventricular systolic function: a study using PCR and electron microscopy
Autor: | Daniel Krsek, Anna Barbara Danek, Dagmar Berenová, Jaroslav Lindner, Ivana Vitkova, Tomas Palecek, Aleš Linhart, Zuzana Kurzová, Petr Kuchynka, Pavla Balatova, Tomas Grus, Eduard Nemecek, Jana Podzimkova |
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Rok vydání: | 2016 |
Předmět: |
Cardiomyopathy
Dilated Male Pathology medicine.medical_specialty Myocarditis lcsh:Medicine Enzyme-Linked Immunosorbent Assay 030204 cardiovascular system & hematology Polymerase Chain Reaction Ventricular Function Left General Biochemistry Genetics and Molecular Biology Serology borrelia burgdorferi 03 medical and health sciences 0302 clinical medicine Western blot myocardium medicine Humans Prospective Studies 030212 general & internal medicine Borrelia burgdorferi Prospective cohort study Aged Lyme Disease Ejection fraction medicine.diagnostic_test biology business.industry lcsh:R Heart Dilated cardiomyopathy biology.organism_classification medicine.disease dilated cardiomyopathy Microscopy Electron cardiovascular system Immunohistochemistry Female myocarditis business |
Zdroj: | Biomedical Papers, Vol 160, Iss 1, Pp 136-139 (2016) |
ISSN: | 1804-7521 1213-8118 |
Popis: | Background: Several studies have demonstrated the presence of the Borrelia burgdorferi (Bb) genome in the myocardium of patients with dilated cardiomyopathy (DCM). To further support a causal relationship between the presence of Bb in the heart muscle and the development of DCM, demonstration of the absence of Bb in the myocardium of subjects with normal left ventricular (LV) systolic function is needed. Aim: To determine the prevalence of Bb by polymerase chain reaction (PCR) and electron microscopy (EM) in individuals with normal LV systolic function and no history suggestive of myocarditis. Methods: We investigated 50 patients (67 ± 9 years, 15 women) with normal LV ejection fraction (EF) ≥ 50% undergoing cardiac surgery. During surgery, four samples from the right atrial appendage were obtained and subsequently examined by PCR and EM for the presence of Bb, and by immunohistochemistry to detect inflammatory cells. Serological testing of antibodies against Bb was also performed. Results: Neither PCR nor EM detected Bb in any of the subjects. Immunohistological examination revealed myocardial inflammation in 2 individuals (4%). Serological analysis by enzyme-linked immunosorbent assay demonstrated IgM antibodies against Bb in 4% and IgG antibodies in 12% of the study cohort; Western blot revealed IgM as well as IgG positivity in 14% of patients. Conclusions: The absence of Bb in the myocardium of individuals who undergo cardiac surgery and have normal LV systolic function supports the idea of Bb pathogenicity in the development of DCM. |
Databáze: | OpenAIRE |
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