Daily sesame oil supplementation mitigates ketoconazole-induced oxidative stress-mediated apoptosis and hepatic injury
| Autor: | Se Ping Chien, Ming Yie Liu, Chuan Teng Liu, Srinivasan Periasamy, Ying Chien Chen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Necrosis Antifungal Agents Endocrinology Diabetes and Metabolism 030106 microbiology Clinical Biochemistry Antifungal drug Apoptosis Pharmacology medicine.disease_cause Biochemistry Antioxidants Neutrophil Activation Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound medicine Animals Molecular Biology chemistry.chemical_classification Liver injury Reactive oxygen species Mice Inbred ICR Nutrition and Dietetics business.industry Glutathione medicine.disease Oxidative Stress 030104 developmental biology Ketoconazole chemistry Liver Dietary Supplements Osteopontin Lipid Peroxidation medicine.symptom Chemical and Drug Induced Liver Injury business Reactive Oxygen Species Oxidation-Reduction Oxidative stress Biomarkers Sesame Oil medicine.drug |
| Zdroj: | The Journal of nutritional biochemistry. 37 |
| ISSN: | 1873-4847 |
| Popis: | Ketoconazole (KCZ) is the most commonly used systemic antifungal drug. However, long-term treatment of KCZ induces hepatic injury. Oxidative stress is involved in KCZ-induced hepatic injury. Oxidative stress plays an important role in apoptosis-associated hepatic damage. Sesame oil is rich in potent antioxidants and antifungal constituents. It attenuates hepatic injury by inhibiting oxidative stress. Thus, sesame oil may protect against KCZ-induced oxidative stress, apoptosis and hepatic damage. The aim of the present study was to investigate the protective effect of sesame oil as a nutritional supplement on KCZ-induced hepatic injury in mice. KCZ (300 mg/kg/day) was administered by gastric intubation; 30 min later, sesame oil (0, 0.0625, 0.125, 0.25 or 0.5 ml/kg/day; p.o.) was administered to mice for 14 days. Blood and liver tissue were collected. Hepatic injury was evaluated by serum biochemistry and histology. Oxidative stress was evaluated by myeloperoxidase activity, p47-phox, reactive oxygen species generation, lipid peroxidation and glutathione level. Apoptosis was evaluated by p53, caspase-3, Bcl-2, Bax and Cyto-C expression. Osteopontin was measured to assess liver healing. Sesame oil attenuated hepatic injury; it also decreased oxidative stress and apoptosis in KCZ-treated mice. Sesame oil may be used as a nutritional supplement with existing antifungal therapies to neutralize the adverse hepatotoxic nature of antifungal drugs by attenuating hepatic apoptosis through redox system to protect and heal liver injury in KCZ-treated mice. |
| Databáze: | OpenAIRE |
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