| Autor: |
Yu-Qi Liu, Min Luo, Yu Shi, Ying Guo, Hua Zhang, Kai-Di Yang, Tian-Ran Li, Liu-Qing Yang, Ting-Ting Liu, Bo Huang, Qing Liu, Zhi-Cheng He, Xiao-Ning Zhang, Wen-Ying Wang, Shuai Wang, Hui Zeng, Qin Niu, Xia Zhang, You-Hong Cui, Zhi-Ren Zhang, Xiu-Wu Bian, Yi-Fang Ping |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Oncogene. 41(30) |
| ISSN: |
1476-5594 |
| Popis: |
Glioblastoma is a lethal primary brain tumor with abundant immune-suppressive glioblastoma-associated macrophage (GAM) infiltration. Skewing immune suppressive GAMs towards an immune-activating phenotype represents a promising immunotherapeutic strategy against glioblastoma. Herein, we reported that genetic deletion of miRNA-processing enzyme Dicer in macrophages inhibited the growth of GL261 murine glioblastoma xenografts and prolonged survival of tumor-bearing mice. Single cell RNA sequencing (scRNA-seq) of the tumor-infiltrating immune cells revealed that Dicer deletion in macrophages reduced the proportion of cell-cycling GAM cluster and reprogramed the remaining GAMs towards a proinflammatory activation state (enhanced phagocytotic and IFN-producing signature). Dicer-deficient GAMs showed reduced level of cyclin-dependent kinases (CDK1 and CDK2) and increased expression of CDK inhibitor p27 Kip1, thus manifesting impaired proliferation. Dicer knockout enhanced phagocytotic activity of GAMs to eliminate GL261 tumor cells. Increased proinflammatory GAM clusters in macrophage Dicer-deficient mice actively interacted with tumor-infiltrating T cells and NK cells through TNF paracrine signaling to create a pro-inflammatory immune microenvironment for tumor cell elimination. Our work identifies the role of Dicer deletion in macrophages in generating an immune-activating microenvironment, which could be further developed as a potential immunotherapeutic strategy against glioblastoma. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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